摘要
应用荧光偏振免疫分析法和时间分辨荧光免疫分析法分别测定血浆同型半胱氨酸（homocysteine，Hcy）和血清叶酸（FA）、维生素B12（VitB12）的水平，探讨其在糖尿病肾病（diabetic nephropathy，DN）中的临床价值。选择137例糖尿病（DM）患者，并根据尿微量白蛋白（uMA）的排泄率及血肌酐（SCr）水平将其分为糖尿病无肾病组、DN初期组、临床DN组和氮质血症组，同时测定以40名健康人作对照组，检测其Hcy、FA和VitB12。采用SPSS10．0统计软件，进行直线回归相关分析。结果显示：四组DM患者血浆Hcy水平分别与对照组比较均显著性增高（P〈0．05～0．01），并随DN患者肾脏损伤程度的加重而升高，每两组间相比有显著性差异（P〈0．01）。四组DM患者血清FA、VitB12水平分别与对照组比较，除糖尿病无肾病组无显著性差异（P〉0．05）外，其余三组均有显著性差异（P〈0．01），每两组间比较均有显著性差异（P〈0．01）并与血浆Hcy水平呈现负相关。结论：高Hcy血症是DM和DN患者的危险因素，血浆Hcy水平可作为DM和DN病情监测的有效指标，FA、VitB12的降低可能是诱发高Hcy血症的一个重要因素。
To determine plasma homocysteine（Hcy）, serum folic acid（FA） and vitamine B12 （VitB12） with fluorescence polarization immunoassay and TRFIA, respectively, and to evaluate their clinical significance on diabetic nephropathy（DN）, 137 patients with diabetes mellitus （DM） were selected and divided into 4 groups according to their excretion rate of urine microalbumine and serum creatinine levels, including group without nephropathy of DM, primary stage of DN group , clinical stages of DN group and azotemia group, and 40 healthy subjects were selected as control group. The results were analysed with SPSS10.0. Results showed that plasma Hey levels in group without nephropathy of DM, primary stage of DN group, clinical stages of DN group and azotemia group were significantly higher than that in control group（P〈 0.05-0.01） and increased with renal damnificantion of DN. It also showed significant difference（P〈0.01） between each group in these four groups. Levels of serum FA, VitB12 were significant different（P〈0.01） in groups of patient with healthy group except group of DM patients without nephroathy（P〉0.05）, and showed significant negative correlation with plasma Hey. So it can be concluded that high level Hey in plasma plays an important risk role in patients with DM and DN, and lower level FA and VitB12 may induce high concentration of Hcy in plasma.
出处
《标记免疫分析与临床》
CAS
2006年第4期207-209,212,共4页
Labeled Immunoassays and Clinical Medicine
