摘要
目的设计一种更贴近人类老年性痴呆(AD)病变特征的新的多因素模型,为研究AD的发病机制和药物开发提供理想的实验模型。方法SD雄性大鼠侧脑室内连续14d注入Aβ1～40,连续5d注入1％氯化铝溶液。首次注射时在丘脑前背侧核注入10ng TGFβ1。分别于术后1周和术后3个月取脑组织进行病理学染色,免疫组化方法检测ChAT和Aβ的活性。结果(1)模型鼠脑组织神经元排列紊乱、核固缩,刚果红染色阳性,3个月后出现老年斑和神经纤维缠结;(2)脑组织内ChAT活性降低,Aβ活性增加;(3)这些特征性改变持续时间长。结论本模型是一种由多种致病因素共同作用的新的、理想的AD动物模型。
Objective To design a new animal model of Alzheimer＇s disease（AD） ,which can more completely mimic the distinctive changes of human AD and will be very helpful in the investigation of pathogenesis and pharmacy of AD in the future. Methods Beta-amyloid 1-40 and aluminum chloride solution were injected into the lateral cerebral ventricle of SD male rats respectively for continuous 14d and 5d,10ng TGFβ1 was injected into the anterodorsal nucleus of thalamus at the first time. Respectively, one week and three months after the operation, the brain tissues of these rats were stained, the activities of their choline acetyhransferase and beta-amyloid were detected with the method of immunohistochemistry. Results （1）The neurons of the sample rats showed derangement, karyopyknosis, and their brain tissues were positive when stained by Congo red. Furthermore, senile plaques and neurofibrillary tangles were found in the cortex and hippocampus 3 months after the operation. （2）The activity of choline acetyl transferase decreased and the beta-amyloid increased. （3）These distinctive changes can last a long time. Conclusion The model is a successful, ideal and new animal model of AD with multiple factors.
出处
《重庆医学》
CAS
CSCD
2007年第2期146-148,151,共4页
Chongqing medicine
关键词
老年性痴呆
动物模型
Β-淀粉样蛋白
铝
转化生长因子Β1
Alzheimer＇s disease
animal model
beta amyloid protein
aluminum
transforming growth factor beta 1
