摘要
对维甲酸预防甲基硝基亚硝基肌（ＭＮＮＧ）诱导体外培养胎胃粘膜上皮细胞恶性转化的作用进行研究。结果发现，胃粘膜细胞先用１０－５Ｍ或１０－６Ｍ维甲酸预处理４ｈ，再给ＭＮＮＧ，细胞增殖速率、软琼脂集落形成能力、非程序ＤＮＡ合成（ＵＤＳ）水平、质脂过氧化物和ｒａｓｐ２１含量均显著低于ＭＮＮＧ组（Ｐ＜０．０５～０．０１）。结果提示，维甲酸对ＭＮＮＧ诱导的胃粘膜上皮细胞损伤有防护作用。
The preventive effects of retinoic acid (RA) on gastric epithelial cells(GEC) in vitro damaged by N methyl N′ nitro N nitrosoguanidine(MNNG)were studied.It was found that the proliferative rate,the number of colony formation in soft agar,the level of unscheduled DNA synthesis(UDS),the contents of lipid peroxidation products and the concentrations of ras p21 in GEC,treated with 10 -5 mol and 10 -6 mol RA for 4h prior to MNNG administration,decreased obviously,compared with GEC treated without RA,the differences were significant( P <0 05～0 01).Our results indicate that RA can effectively prevent chemical damage on GEC from MNNG.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1996年第5期333-335,共3页
Medical Journal of Chinese People's Liberation Army
