摘要
目的:观察两种维甲酸( RA) 对人胰腺癌细胞生长的抑制作用及其可能机制。方法:应用MTT 比色法、流式细胞仪、裸鼠肾包膜下移植瘤抑制试验和透射电镜技术进行检测。结果:RA 抑制胰腺癌细胞生长,6 d 组抑制50 %细胞生长的药物浓度(IC50) 全反式RA(ATRA) 和13 - 顺式(CRA) 分别为10 ~30 μmolL 和30 ~50 μmolL。癌细胞在RA作用下,G0/G1 期细胞比例增加,S 期细胞比例和增殖指数(PI) 下降。ATRA 6 d 组凋亡指数( AI) 增高,ATRA 和CRA 6 d组AIPI 比例分别是对照组的4 倍和2 倍。ATRA 能显著抑制肾包膜下胰腺癌移植瘤生长,电镜检查证实部分胰腺癌细胞具有凋亡特征。结论:RA
AIM:To investigate the mechanisms of two kinds of retinoic acid (RA) on the growth inhibition of human pancreatic carcinoma cells. METHODS:MTT colorimetric assays, flow cytometric analysis, subrenal capsule(SRC) xenograft assay in nude mice and transmission electron microscopy were performed. RESULTS:RA inhibited the growth of pancreatic caricinoma cells. The 50% inhibitory concentration (IC 50 ) of all-trans RA(ATRA) and 13-cis RA(CRA) on cell growth occurred after 6d induction were 10~30 μmol/L and 30~50 μmol/L respectively. RA increased the precentage of cell population in the G 0/G 1 phase ,but reduced it in S phase and also reduced proliferation index (PI). Cell apoptosis index (AI) was higher in 6d ATRA treated group than control group. AI/PI of ATRA and CRA 6d treated group were 4 and 2 times as control group. ATRA inhibited significantly growth of SRC pancreatic carcinoma. The morphology of ATRA treated cells was primarily characterized by apoptosis. CONCLUSION:The antitumor effects of RA are primarily related to the inhibition of proliferation of pancreatic carcinoma cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第8期746-749,共4页
Chinese Journal of Pathophysiology