摘要
目的探索细胞DNA辐射损伤后离体修复与整体修复的关系,拟合修复曲线,在辐射生物剂量估算中结合应用剂量-效应关系曲线,提高剂量估算的准确性。方法用中性单细胞凝胶电泳技术检测1 Gyγ射线辐照后不同时间点的小鼠和人淋巴细胞DNA双链断裂,分别拟合DNA修复曲线,并对二者曲线模型进行比较。结果人外周血和小鼠辐照后,随照后时间的推移,DNA损伤的修复增加,残余损伤逐渐减少,呈明显的时相-效应关系。人淋巴细胞辐照后体外修复曲线方程模型与动物体内修复曲线方程模型均以对数方程为最佳,分别为：小鼠：Y_(TM)=55．8256-10．792 InX(R^2 =0．629,P＜0．01)和Y(OTM)=25．4173-4．5273 InX(R^2=0．661,P＜0．01)；人：Y_(TM)=30．2427- 7．3836 InX(R^2=0．686,P＜0．01)和Y_(OTM)=17．9772-3．9125 InX(R^2=0．752,P＜0．01)。结论人淋巴细胞离体辐照后的修复过程可基本反映体内DNA双链断裂的修复水平与速度,修复曲线可以作为辐射生物剂量估算时的参考。
Objective To explore the relationship between DNA repair in vitro and in vivo after irradiation, and to describe the curves of DNA repair which can improve the accuracy of radiation dose estimation. Methods The DNA double-strand break in lymphoeytes of human and mouse was detected using neutral single cell gel electrophoresis（SCGE） after radiation and the curves of DNA repair individually were estimated, which were compared later. Results Along with the time lapsing, the DNA repair of human peripheral blood and mice increased significantly and the residual damage decreased gradually, which showed significant time-effect relationship. The curve of DNA repair in vitro of human lymphoeytes presented the same log model as that of mouse DNA repair in vivo . The curve showed as followed respectively： Mice： Y TM= 55.825 6- 10.792 lnX （ R^2 = 0.629, P 〈 0.01 ） and YOTM=25.417 3-4.527 3 lnX （R2 =0.661, P 〈 0.01）;Human： Ya~ 30.242 7-7.383 6 lnX （R^2= 0.686, P 〈 0.01） 和 Y OTM= 17.977 2 - 3.912 5 lnX （ R^2 = 0.752, P 〈 0.01 ）. Conclusion The curve of DNA repair in vitro of human lymphocytes could be considered in biodosimetry estimation because the process of DNA repair in vitro could display the repair level and speed of DNA double-strand break in vivo.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2006年第12期734-738,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
教育部重点学科内科学(辐射血液学)基金资助
天津市自然科学基金资助(043610011)
