摘要
目的:探讨中波紫外线(UVB)造成HaCaT细胞损伤的机制及硫酸锌能否拮抗UVB对细胞的损害作用,为开发预防UVB损伤的药物提供理论依据。方法:将HaCaT细胞随机分为对照组、UVB组、加锌(Zn)组和Zn+UVB组。对照组不施加任何处理因素;UVB组细胞进行UVB照射;Zn组只加入终浓度为50μmol.L-1的硫酸锌;Zn+UVB组,加入硫酸锌24h后,进行UVB照射。UVB照射时间为6min,照射后24h,收集各组细胞,采用台盼蓝拒染法检测细胞存活率,TBA显色法检测细胞内丙二醛(MDA)水平,单细胞凝胶电泳技术检测各组细胞DNA的损伤情况。结果:与对照组比较,UVB组和Zn+UVB组HaCaT细胞存活率显著降低(P<0.05),MDA水平显著升高(P<0.05);与UVB组比较,Zn+UVB组细胞存活率显著升高(P<0.05),MDA水平显著降低(P<0.05)。单细胞凝胶电泳检测结果显示,UVB组和Zn+UVB组HaCaT细胞均出现彗星样拖尾现象。与对照组比较,UVB组和Zn+UVB组细胞尾部DNA尾距、Olive尾距显著增大(P<0.05);与UVB组比较,Zn+UVB组尾距、Olive尾距显著减小,差异有统计学意义(P<0.05)。结论:UVB能够导致HaCaT细胞存活率下降和MDA水平提高以及DNA损伤,而硫酸锌能够拮抗UVB对HaCaT细胞的损伤作用。
Objective To study the antagonism of zinc sulfate on damage effect of ultraviolet B（UVB） on HaCaT cells,and to provide theoretical basis for development of drug to prevent UVB damage.Methods The HaCaT cells were cultivated in RPMI 1640 and randomly divided into control group,Zn group,UVB group and Zn＋UVB group.Nothing was administrated to the cells in control group,the cells in UVB group were radiated by UVB,the cells in Zn group were administrated with 50 μmol·L-1 ZnSO4 and the cells in Zn＋UVB group were administrated with 50 μmol·L-1 ZnSO4 24 h before UVB radiation.The UVB radiation time was 6 min,the cells in every group were collected 24 h after UVB radiation.The cell viability was measured by trypan blue exclusion assay,the MDA level was measured by TBA method,the DNA damage in HaCaT cells was measured by single cell gel electrophoresis（SCGE）.Results Compared with control group,24 h after radiation,the cell viabilities in UVB group and Zn＋UVB group were significantly decreased（P〈0.05）,the MDA levels were increased（P〈0.05）.Compared with UVB group,the cell viability in Zn＋UVB group was significantly increased（P〈0.05）,the MDA level was decreased（P〈0.05）.The SCGE results revealed that the DNA fragmentation（comet） of the cells in UVB group and Zn＋UVB group were induced by UVB.The tail extent moment and Olive tail moment of the cells in UVB group were significantly higher than those in Zn＋UVB group（P〈0.05）.Conclusion UVB can lead the decreasing of the viability of HaCaT cells,the increasing of MDA level and DNA damage,while zinc sulfuric can antagonize the damage effect of UVB on HaCaT cells.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2011年第4期646-650,774,共6页
Journal of Jilin University：Medicine Edition
基金
中华医学会欧莱雅基金资助课题(S-2007040803)
吉林大学大学生创新基金资助课题(2010A72111)
