摘要
目的探讨整体照射与离体照射导致小鼠淋巴细胞DNA双链断裂的一致性,作为单细胞凝胶电泳技术应用于辐射生物剂量学的前期研究。方法采用双层铺胶法进行中性单细胞凝胶电泳,观察小鼠淋巴细胞整体与离体照射后的DNA双链断裂,用CASP软件分析彗星图像,SPSS12.0进行数据的统计学分析。结果彗星头部DNA%(HDNA%)、尾部DNA%(TDNA%)、彗星全长(CL)、尾长(TL)、尾矩(TM)和Olive尾矩(OTM)在整体照射和离体照射组之间的差别均无显著性。结论离体血γ射线照后即刻进行中性单细胞凝胶电泳,可以客观准确地反映整体照射的淋巴细胞DNA双链断裂损伤。
Objective To observe the consistency of DNA double-strand break between in vivo and in vitro irradiation, as a prophase study in radiation biodosimetry using single cell gel electrophoresis（SCGE）. Methods Detect DNA double-strand break after whole-bedy and in vitro radiation in mice lymphocytes using neutral single cell gel electrophoresis. The comet images were processed by CASP software and all the data were analysed by SPSS12.0. Results There is no difference between in vivo and in vitro irradiation group in HDNA% ,TDNA% ,CL,TL,TM and OTM. Conclusion The result of neutral single cell gel electrophoresis shortly alter in vitro irradiation can precisely reflect the DNA double-strand break of lymphocytes in whole-bedy irradiation.
出处
《中国辐射卫生》
北大核心
2006年第2期153-155,共3页
Chinese Journal of Radiological Health
基金
教育部重点科学内科学(血液学)基金资助
天津市自然科学基金资助项目(043610011)
关键词
单细胞凝胶电泳
DNA双链断裂
整体照射
离体照射
Single Cell Gel Electrophoresis
DNA Double-strand Break
Vivo Irradiation
Vitro Irradiation
