摘要
目的研究固体培养条件下一种真菌诱导子对铁皮石斛原球茎生长及总生物碱含量的影响。方法在铁皮石斛原球茎固体培养的后期加入内生真菌诱导子,并以加入等量葡萄糖溶液、麦麸培养基、蒸馏水为对照,考察真菌诱导子对原球茎鲜重、干重及总生物碱含量的影响。结果与加蒸馏水的对照相比,真菌诱导子、对照的麦麸培养基和葡萄糖溶液均可以极显著性地提高铁皮石斛原球茎的干重(P<0.01),分别提高了35.7%,34.3%和22.7%;并显著性地提高原球茎的折干率达20.7%,20.7%和16.3%(P<0.05)。与加真菌诱导子的原球茎相比,附加等量麦麸培养基和葡萄糖溶液培养的原球茎总生物碱含量分别降低了23%和17%。结论在铁皮石斛原球茎固体培养的后期加入真菌诱导子能促进原球茎的生长,并对总生物碱含量有一定的影响。
OBJECTIVE To study the effect of a fungal elicitor on the growth and the total alkaloid content of Dendrobium candidum protocorms cultured on the solid medium. METHODS D. candidum protocorms were cultured on the solid medium for 14 weeks, and respectively added fungal elicitor （E） and three comparisons which were wheat bran culture medium （M）, glucose solution （G） and distilled water （W） for 6 weeks. The fresh weight and dry weight of harvest protocorms and their total alkaloid content were measured, RESULTS Compared with W, E, M and G significantly increased the dry weight of protocorms by 35.7%, 34.3% and 22.7%, respectively （ P 〈 0.01）, and enhanced the dry weight coefficient of protocorms by 20.7 %, 20.7% and 16.3 %, respectively （P 〈 0.05）. Compared with that of E, the total alkaloid content of protocorms which were cultured on control M and Control G decreased by 23% and 17% ,respectively. CONCLUSION The growth of D. candidum protocorms can be improved when they are cultured on the solid medium added the fungal elicitor at the later stage of culture, and their total alkaloid content can also be affected.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第22期1692-1694,共3页
Chinese Pharmaceutical Journal
基金
国家杰出青年科学基金专项(30325047)
关键词
铁皮石斛原球茎
真菌诱导子
生长
总生物碱含量
Dendrobium candidum protocorm
fungal elicitor
growth
total alkaloid content
