摘要
以黑曲霉提取物作为真菌诱导子,研究其对提高喜树悬浮培养细胞的生长和喜树碱产量的影响。结果表明:终浓度40 mg/L的诱导子在细胞培养的第14天加入,细胞干重在第26天达到最大量31.5 g/L,是未处理的细胞干重的1.2倍;喜树碱产量在第29天达到最大值13.6mg/L,是对照产量的6.0倍,没有受到诱导处理的细胞干重和喜树碱产量都在第20天达到最大值。与真菌诱导子刺激提高细胞生长和喜树碱生物合成相对应的是,诱导刺激的培养细胞表现出较高的细胞活力,培养基中糖类的消耗量也随之增加。
A fungal elicitor from Aspergillus niger could significantly stimulate cell growth and camptothecin biosynthesis with suspension cultures of Camptotheca acuminate Decaisne.When the elicitor at 40 mg/L of final concentration was supplemented with suspension cultures on the 14th day of growth,the highest dry cell weight（31.5 g/L） was achieved on the 26th day,recording about 1.2-folds increase over that of untreated cells.Maximum camptothecin yield（13.6 mg/L） was achieved on the 29th day,which was about 6.0-folds higher than that of the control.Maximum cell dry weight and camptothecin yield of unelicited cells were both obtained on day 20.Corresponding to the higher cell growth rate and increased alkaloids acumination induced by the fungal elicitor,higher cell viability and enhanced consumption rates of sugar occurred in elicited cells during subsequent cell suspension subcultures of C.acuminata.
出处
《北方园艺》
CAS
北大核心
2010年第20期156-159,共4页
Northern Horticulture
基金
湖北省自然科学基金资助项目(2006ABA323)
武汉大学博士后研究基金资助项目[2008(205-180516)]
关键词
真菌诱导子
喜树
喜树碱
黑曲霉
植物细胞培养
fungal elicitor
Camptotheca acuminata decaisne
camptothecin
Aspergillus niger
plant cell culture
