摘要
从含油废水处理曝气池活性污泥中分离到具有絮凝特性的絮凝菌株F2、F6,絮凝率在80%以上.对絮凝菌F2、F6的絮凝基因进行基因定位.首先对菌株F2、F6进行药物抗性遗传标记选择,然后提取质粒,其中F6无质粒,则絮凝基因在染色体上;F2有质粒,将具有絮凝特性的菌株F2的质粒转化到无絮凝特性的受体菌DH5α细胞中,最后对转化菌进行絮凝特性检测.结果表明,转化菌株的絮凝效果明显低于原始亲株F2.初步判断F2、F6絮凝基因定位于染色体DNA上,不在质粒上.因此,为了提高絮凝菌的絮凝效果,利用高效产絮菌株F2、F6作为亲本菌株,进行原生质体融合,对融合条件进行优化,并对融合子进行絮凝测定.实验结果确定了青霉素GK的最适浓度,F2为0.6U·mL-1、F6为0.1U·mL-1,得到了相应的原生质体及融合子;经絮凝试验对融合子的验证,结果表明,数株融合子表现出产絮特性,融合子絮凝效果与亲本菌株F2、F6相当.
The strain F2, F6 with high flocculating activity was isolated from activated sludge of oil wastewater treatment system. The flocculation efficiency is more than 80%. The flocculating gene of strain F2 ,F6 was located and then antimicrobial tolerance genetic marker was selected. The plasmid extracted from F2 was transformed to recipient DHSα without flocculating activity. The flocculation characteristics of transformants were detected. The results showed that the flocculating activity of the transmitted isolates was obviously lower than that of parental strains F2. It could be concluded preliminarily that flocculant gene located on chromosomes DNA and not on plasmid. In order to improve their flocculation efficiency, cell-fusion technique is adopted to investigate the capacity of flocculation producing bacteria. Protoplast of the bacteria is the selection fusion target. The optimal operating conditions for cell-fusion are investigated, as well as the detection of syncretized cell. The experiments results showed that the best concentration of penicillin GK for strain F2 is 0.6 U·mL^-1 and for strain F6 is 0.1U·mL^-1. Correspondingly protoplast and fusant are obtained. The syncretized cells has the characteristics of flocculation production and their flocculation capacities are similar to strain F2 ,F6.
出处
《环境科学学报》
CAS
CSCD
北大核心
2006年第12期1994-1999,共6页
Acta Scientiae Circumstantiae
基金
国家重点基础研究计划项目(973)(No.2004CB418505)~~
