摘要
[目的]探讨GSTM1和GSTT1基因多态性与外周血淋巴细胞DNA损伤的关系。[方法]选择某焦化厂248名焦炉作业工人,其中炉顶区作业工人99人,炉侧作业78人,炉底作业71人。120名与之相匹配的对照人群选自该厂非职业多环芳烃(PAHs)暴露者。采用单细胞凝胶电泳实验观察外周血淋巴细胞DNA损伤;运用多重PCR方法检测GSTM1、GSTT1基因多态性。同时,通过问卷调查的方式了解被调查者的年龄、吸烟、饮酒和个人职业暴露史。[结果]炉顶和炉侧区作业工人外周血淋巴细胞的Olive尾矩(OliveTailMoment,OTM)经对数转换后分别为1.37±1.16和1.46±0.97,均明显高于炉底区和对照组(OTM分别为0.98±1.18、0.56±0.99),差异显著(P<0.05)。经调整年龄、吸烟、暴露等级等因素后,焦炉作业组中GSTM1(-)基因型和GSTM1(+)基因型的外周血淋巴细胞DNA损伤分别为1.36±1.15和1.15±1.10,差异无显著性(P>0.05)。其中炉顶区作业工人GSTM1(-)基因型OTM明显高于GSTM1(+)基因型(分别为1.56±1.08、1.09±1.25,P<0.05)。此外,在焦炉作业组中,GSTM1(-)GSTT1(+)基因型OTM为1.44±1.13,明显高于GSTM1(+)GSTT1(+)基因型0.94±1.06,差异具有显著性(P<0.05);GSTM1(+)GSTT1(-)和GSTM1(-)GSTT1(-)基因型OTM分别为1.36±1.10、1.28±1.17,两者差异无显著性。[结论]在炉顶作业工人中,GSTM1基因型可以明显影响外周血淋巴细胞DNA损伤程度。GSTM1和GSTT1基因存在交互作用,同时携带GSTM1(+)和GSTT1(+)基因型的焦炉作业工人淋巴细胞DNA损伤水平较低。
[ Objective ] To investigate the association between genetic polymorphisms of glutathione S-transferase （ GST ）and DNA damage in lymphocytes among coke oven workers. [ Methods ] 248 coke oven workers and 120 controls without occupational polycyclic aromatic hydrocarbons（ PAHs ）exposure were recruited in this study. The coke oven workers were divided into three subgroups： i.e. top of coke-oven, side of the oven and ground of the oven, based on the external occupational exposure level. Single cell gel electruphoresis was used to evaluate DNA damage, and the genotype of GSTM1 and GSTT1 were determined by multiplex PCR method. Individual information including age, smoking, addiction to alcohol status and occupational exposure history was collected by questionnaire. [ Results] The lymphocytes DNA damage level in top of oven group and side of cokeoven group were 1.37 ± 1.16 and 1.46 ± 0.97, which were significantly higher than that of ground of oven group and controls （ 0.98 ± 1.18 and 0.56 ± 0.99, P 〈 0.05 ）. After standardized by age, smoking and external occupational exposure level, the OTM of coke oven workers with null genotype at GSTMI gene was 1.36 ± 1.15, which was higher than that of those with non-null genotype（ 1.15 ± 1.10, P 〉 0.05 ）with no statistically significance. However, in the top of oven group, there was significant difference between null and non-null genotype at GSTMI（ 1.56 ± 1.08 vs 1.09 ±1.25, P 〈 0.05 ）. The results also show that the OTM with GSTMI（ - ） GSTTI （ ＋ ）genotype was obviously higher than those with GSTMI（ ＋ ）GSTTI（ ＋ ）genotype among coke oven workers （ 1.44 ± 1.13 vs 0.94 ± 1.06, P 〈 0.05 ）. The OTM of coke oven workers with GSTM1 （ ＋ ） GSTT1 （ - ） and GSTM1 （ - ） GSTT1 （ - ） genotypes were 1.36± 1.10 and 1.28 ± 1.17, which were higher than that of the individuals with GSTMI（ ＋ ）GSTTI（ ＋ ） genotype with no significance statistically. [ Conclusion ] The genotype of GSTM1 was associated w
出处
《环境与职业医学》
CAS
北大核心
2006年第6期492-495,共4页
Journal of Environmental and Occupational Medicine
基金
国家重点基础研究规划项目(编号:2002CB512905)
