摘要
目的研究多环芳烃(PAHs)暴露(环境空气暴露和吸烟)、GSTT1、GSTM1基因型对1-羟基芘(1-OHP)作为PAHs暴露标志物的影响。方法选取51名巡警作为研究组,48名内勤警察作为对照组,测定两组人群环境空气中的PAHS浓度以及尿中的1-OHP浓度,采用HPLC方法分析环境空气中PAHs浓度和研究对象尿样中的1-OHP;PCR方法测定GSTT1、GSTM1基因型。结果对照组环境PAHs浓度为12.79ng/m3,研究组为20.85ng/m3。研究组、对照组内部相同吸烟条件下GSTT1、GSTM1不同基因型人群尿中1-OHP浓度没有差别,相同基因型下的非吸烟人群中,对照组尿中1-OHP浓度均小于研究组,吸烟人群中没有发现同样的规律。按吸烟分层后,研究组、对照组内部吸烟者尿中的1-OHP浓度均大于非吸烟者,并且对照组吸烟者尿中1-OHP浓度大于研究组非吸烟者。结论PAHs暴露及吸烟是影响尿中1-OHP浓度的重要因素;在环境空气中PAHs浓度较低的情况下,吸烟对尿中1-OHP浓度的贡献更大。但GSTT1、GSTM1基因型不是影响尿中1-OHP浓度的主要因素。
Objective To study the influence of polycyclic aromatic hydrocarbons(PAHs) exposure (ambient concentration and smoking) and GSTT_1,GSTM_1 genotypes on urinary 1-OHP as exposure biomarker.Methods 51 Cops who working outside were selected as study group,48 Cops who working in office were selected as control Group.The ambient concentrations of PAHs both of two circumstances and the urinary 1-OHP of subjects were measured.The ambient concentrations of PAHs and the urinary 1-OHP were determined by high performance liquid chromatography (HPLC).The GSTT_1,GSTM_1 genotypes were determined by PCR.Results The ambient concentrations of PAHs in the study group and the control group were 20.85ng/m^3 and 12.79 ng/m^3 respectively. There were not significant difference in urinary 1-OHP among the persons who had the deference GSTT_1,GSTM_1 genotypes and the same smoking habits in the study group and the control group.The urinary 1-OHP concentrations of the study group were higher than those of the control group when they had the same genotypes for non-smokers. Smoking stratified analysis showed that urinary 1-OHP concentrations of smokers were higher than those of non-smokers, the urinary 1-OHP concentrations of smokers in control group were higher than those of the non-smokers in study group.Conclusion The ambient PAHs concentration and smoking could be the important factors generated the urinary 1-OHP concentration. While the levels of ambient PAHs were low, the smoking could change more prominent. The GSTT_1,GSTM_1 genotypes could not be the major influential factors.
出处
《卫生研究》
CAS
CSCD
北大核心
2009年第3期261-264,共4页
Journal of Hygiene Research
基金
国家自然科学基金资助项目(No.40575064)
关键词
环境空气暴露
吸烟
1-羟基芘
GSTT1
GSTM1
多环芳烃
ambient concentration, smoking, urinary 1-OHP concentration, GSTT_1,GSTM_1, polycyclic aromatic hydrocarbons
