摘要
目的:探讨中药黄芪对兔内毒素性急性肺损伤(AL I)的保护作用。方法:将12只雄性新西兰兔采用压力控制辅助通气模式后静脉注射脂多糖,当氧合指数(PaO2/F iO2)≤300 mm Hg(1 mm Hg=0.133 kPa)时认为AL I模型制备成功。此时将动物随机分为黄芪组和对照组,每组6只。黄芪组静脉注射黄芪注射液8 g/kg;对照组静脉注射等量生理盐水。观察AL I时以及AL I后1、2、3、4、5和6 h肺动态顺应性(Cdyn)、平均动脉压(M AP)的变化。于AL I后6 h活杀动物取肺组织,观察过氧化物歧化酶(SOD)活性和丙二醛(M DA)含量的变化以及肺组织病理变化。结果:对照组AL I后各时间点肺Cdyn较基础值显著降低,AL I后5 h和6 h M AP较基础值显著下降,差异均有显著性(P<0.05或P<0.01);但两组间肺Cdyn和M AP的变化差异无显著性。黄芪组肺Cdyn及M AP组内比较差异无显著性;黄芪组SOD活性较对照组升高,M DA含量较对照组降低,差异均有显著性(P<0.01和P<0.05);光镜下黄芪组肺损伤组织病理变化较对照组减轻。结论:黄芪对兔内毒素性AL I有明显的保护作用。
Objective： To study the protective effect of radix astragali （黄芪） on acute lung injury （ALI） induced by lipopolysaccharide （LPS） in rabbits. Methods： ALI model was established by ventilation with assistance of pressure control followed by intravenous injection of LPS. When the partial pressure of oxygen in artery/fractional concentration of inspired oxygen （PaO2/FiO2） reduced to≤ 300 mm Hg （1 mm Hg 0.133 kPa）, the model was established successfully. Twelve male New Zealand rabbits were randomly divided into two groups： radix astragali group and control group （each n = 6）. Intravenous infusion of radix satragali injection （8 g/kg） was applied in the radix astragali group. An equal volume normal saline was used in the control group. The changes of mean arterial pressure （MAP） and lung dynamic compliance （Cdyn） were observed at basic situation, onset of ALI, 1, 2, 3, 4, 5 and 6 hours after ALI. The animals were sacrificed 6 hours after ALI. Superoxide dismutase （SOD） activity, malondialdehyde （MDA） content, and histomorphology of lung tissue were examined 6 hours after ALI. Results： Lung Cdyn was obviously decreased at each time point compared with the basic value in control group. MAP was obviously decreased at 5 and 6 hours after ALI compared with the basic value in control group （P〈0. 05 or P〈0.01）. There were no differences of lung Cdyn and MAP between two groups at each time point. In the radix astragali group, the comparisons of lung Cdyn and MAP had no differences, SOD activity in the radix astragali group was significantly higher than that in control group （P〈0.01）, while the content of MDA in the radix astragali group was lowered compared with that in control group （P〈0.05）. The pathologic changes of lung tissue under light microscope in the radix astragali group were lighter than that in control group. Conclusion： Infusion of radix astragali has protective effect on ALI induced by LPS in rabbits.
出处
《中国中西医结合急救杂志》
CAS
2006年第6期348-350,F0003,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
关键词
黄芪
兔
内毒素
肺损伤
急性
radix astragali
rabbit
lipopolysaccharide
acute lung injury
