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姜黄素预先给药对兔呼吸机相关性肺损伤时Nrf2蛋白表达的影响 被引量:7

Effects of curcumin pretreatment on expression of Nrf2 protein during ventilator-induced lung injury in rabbits
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摘要 目的 评价姜黄素预先给药对兔呼吸机相关性肺损伤核因子E2相关因子2(Nrf2)蛋白表达的影响.方法 健康成年雄性新西兰大白兔24只,3~6月龄,体重2.5 ~ 3.0 kg,采用随机数字表法分为3组(n=8):双肺通气组(TLV组)、单肺通气组(OLV组)和姜黄素预先给药组(Cur组).于通气前7d开始灌胃给药,Cur组给予姜黄素40 mg/kg(溶于2ml 1%羟甲基纤维素钠),TLV组和OLV组给予等量羟甲基纤维素钠,每天早晚各1次.行气管切开术,TLV组采用单腔气管导管置入气管行双肺通气,OLV组和Cur组采用单腔气管导管置入右主支气管行单肺通气.3组均采用容量通气模式,调整通气参数,维持SpO2>90%.于通气开始即刻(T0)、通气1、2和3 h(T1-3)时采集右股动脉血样行血气分析,测定PaO2,计算氧合指数.通气3h时处死兔,取右肺称湿重,然后取下叶肺组织,采用化学比色法测定肺组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性;光镜下观察肺组织病理学结果并行肺组织病理学损伤评分,Western blot法检测Nrf2和HO-1蛋白表达,将右肺烘烤后称干重,计算右肺湿/干重比值(W/D比值).结果 与TLV组比较,OLV组和Cur组W/D比值、肺损伤评分、MDA含量、Nrf2和HO-1蛋白表达水平升高,SOD活性和T23时氧合指数降低(P<0.05);与OLV组比较,Cur组W/D比值、肺损伤评分、MDA含量下降,SOD活性、T3时氧合指数、Nrf2和HO-1蛋白表达水平升高(P< 0.05).结论 姜黄素预先给药通过促进肺组织Nrf2蛋白表达减轻兔呼吸机相关性肺损伤. Objective To evaluate the effects of curcumin pretreatment on the expression of Nrf2 protein during ventilator-induced lung injury in rabbits.Methods Twenty-four healthy male New Zealand white rabbits,aged 3-6 months,weighing 2.5-3.0 kg,were randomized into 3 groups (n =8 each) using a random number table:two-lung ventilation (TLV) group; one-lung ventilation (OLV) group; and curcumin pretreatment group (group Cur).In group Cur,curcumin 40 mg/kg (dissolved in 2 ml of 1% sodium carboxymethylcellulose) was given via a gastric tube into the stomach twice a day for 7 consecutive days starting from 7 days before ventilation,while the equal volume of sodium carboxymethylcellulose was given via a gastric tube instead of curcumin in TLV and OLV groups.All the rabbits were tracheostomized,and a tracheal tube was inserted to perform TLV in TLV group,and a tracheal tube was inserted into the right bronchus to establish OLV in OLV and Cur groups.Volumecontrolled ventilation was used in the three groups and the ventilatory parameters were regulated to maintain SpO2 〉 90 %.Immediately before beginning of ventilation (T0) and at 1,2 and 3 h of ventilation (T1-3),arterial blood samples were obtained for blood gas analysis and determination of PaO2.The oxygenation index was calculated.At the end of ventilation,the rabbits were sacrificed and right lungs were removed for determination of wet/dry lung weight ratio (W/D ratio).The right lower lobe was isolated and puhmonary specimens were obtained for determination of malondialdehyde (MDA) content and superoxide dismutase (SOD) activity (using colorimetric method) and the expression of Nrf2 and HO-1 protein (by Western blot) in lung tissues and for microscopic examination of pathological changes of the lung which were scored.Results Compared with group TLV,the W/D ratio,pathological scores,MDA content,and expression of Nrf2 and HO-1 were significantly increased,and the SOD activity and oxygenation index at T2,3 were decreased in OL
出处 《中华麻醉学杂志》 CAS CSCD 北大核心 2014年第2期237-240,共4页 Chinese Journal of Anesthesiology
关键词 姜黄素 呼吸 人工 呼吸窘迫综合征 成人 NF-E2相关因子2 Curcumin Respiration,artificial Respiratory distress syndrome,adult NF-E2-related factor 2
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