摘要
目的：观察补肾活血法组方对顺铂诱导的急性肾损伤（AKI）小鼠的治疗作用。方法选择雄性BALB/c小鼠21只，按随机数字表法分为对照组、AKI模型组、补肾活血法组方治疗组，每组7只。采用单次腹腔注射顺铂20 mg/kg的方法复制AKI模型；治疗组给予补肾活血法组方10μL · g^-1· d^-1，AKI模型组和对照组给予等体积生理盐水灌胃；各组均连续给药6d。观察各组小鼠体质量的变化；收集各组小鼠静脉血，检测血肌酐（SCr）水平；取肾组织观察肾质量，并于光镜下观察肾组织病理学变化，计算急性肾小管坏死评分（ATN）。结果与对照组比较，AKI模型组体质量、肾质量显著降低〔体质量（g）：17.18±0.29比19.33±1.43，肾质量（g）：0.28±0.01比0.32±0.11，均P＜0.01〕，SCr、ATN评分均显著升高〔SCr（μmol/L）：86.77±10.97比14.37±0.81，ATN评分（分）：3.33±0.52比0.17±0.41，均P＜0.01〕；与AKI模型组比较，治疗组体质量（g：18.70±0.28）、肾质量（g：0.31±0.01）显著升高，SCr（μmol/L：21.98±5.52）、ATN评分（分：2.00±0.63）显著下降（均P＜0.01）。光镜下可见：AKI模型组小鼠肾小管上皮细胞水肿、坏死，肾间质有炎性细胞浸润，肾小球形态大致正常；治疗组小鼠肾小管坏死程度较AKI模型组明显减轻，肾小球大致正常。结论补肾活血法组方能降低顺铂诱导的AKI模型小鼠的SCr，改善其肾组织的病理学改变，对AKI有治疗作用。
Objective To investigate the therapeutic effect of traditional Chinese medicine（TCM）recipe of nourishing kidney and activating blood for treatment of cisplatin induced acute kidney injury（AKI）in mouse model. Methods Twenty-one male BALB/c mice were randomly divided into three groups：control group,AKI model group and treatment group with the above TCM recipe（each n=7）. The AKI model was reproduced by a single intraperitoneal injection of cisplatin 20 mg/kg;the TCM recipe with dosage of 10μL · g^-1 · d^-1 was given to the treatment group,while equal volume of normal saline was given to the model and control groups by gavage,lasting for 6 days in all groups. The changes of each mouse body weight were observed;mouse venous blood serum creatinine（SCr）level was detected,changes of renal tissue weight and its histopathology were observed under light microscope,and the score of acute tubular necrosis（ATN）was calculated. Results Compared to the control group,the body and renal weight in AKI model group were significantly lowered〔body weight（g）：17.18±0.29 vs. 19.33±1.43,renal weight（g）：0.28±0.01 vs. 0.32±0.11,both P〈0.01〕,and SCr and ATN scores were significantly increased in AKI model group〔SCr（μmol/L）：86.77±10.97 vs. 14.37±0.81,ATN score：3.33±0.52 vs. 0.17±0.41,both P〈0.01〕. Compared to AKI model group,the body weight（g：18.70±0.28）and renal weight（g：0.31±0.01）in the treatment group were markedly increased,and SCr（μmol/L：21.98±5.52）and ATN scores（2.00±0.63）were significantly lower than those of the AKI model group（all P〈0.01）. Under optical microscope,there were mouse renal tubular epithelial cell edema and necrosis,renal interstitial inflammatory cell infiltration,the glomerular form roughly normal in the AKI model group;compared with the AKI model group,in the treatment group the degree of mouse renal tubular necrosis was significantly reduced and glomerular form was basically normal. Conclusion The TCM recipe of nourishin
出处
《中国中西医结合急救杂志》
CAS
北大核心
2014年第6期405-407,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
“十一五”国家科技支撑计划（2007BA110801-006）
关键词
补肾活血法
顺铂
肾损伤
急性
BALB/C小鼠
中医药治疗
Nourishing kidney and activating blood recipe
Cisplatin
Acute kidney injury
BALB/c mouse
Chinese medicinal therapy
