摘要
目的探讨慢性乙肝患者Th1/Th2类细胞因子变化的意义。方法采用双抗夹心ELISA法对34例慢性乙肝患者发病时和治疗后肝功能恢复正常时外周血Th1类细胞因子(IL-2、IFN-γ)及Th2类细胞因子(IL-4、IL-10)进行检测。与此同时检测血清HBV-DNA。结果与正常对照组相比,IL-2显著下降,下降程度在慢肝轻度<慢肝中度<慢肝重度(P<0.05和0.001);IL-10在慢肝中、重度显著升高(P<0.01),升高程度在慢肝重度>慢肝中度>慢肝轻度,治疗后慢肝中度和慢肝重度较治疗前显著下降(P<0.05)。在慢肝中、重度HBVDNA量治疗后较治疗前显著减少。IL-2、IFN-γ及IL-4、IL-10的水平变化与HBVDNA量无显著相关性。结论慢性乙型肝炎发病时Th2细胞功能增强,且病情越重,Th2细胞功能越强;肝功恢复正常后,Th2细胞功能下降,HBVDNA量减少。
Objective To study the changes and clinical significance of Th1/Th2-type cytokines in patients with chronic hepatitis（CH） B. Methods Blood samples were collected from 34 patients with chronic hepatitis B when the patients were admitted to hospital, another blood samples the liver function of these patients was normal Serum levels of Thl cytokines（ IL-2, IFN-γ） and Th2 cytokines（ IL-4, IL-10 ） were measured by double-antibody sandwich ELISA. Results Compared with the normal control, IL-2 decreased significantly, and CH- Ⅰ 〉 CH- Ⅱ 〉 CH Ⅲ ; IL-10 increased significantly in CH-Ⅱ and CH-Ⅲ ,and CH-Ⅲ 〉 CH-Ⅱ〉 CH- Ⅰ. After the treatment, serum levels of IL-10 and HBV DNA deceased significantly in CH- Ⅱ and CH- Ⅲ. Serum levels of IL-2, IFN-γ and IL-4, IL-10 were no related to the HBV quantity. Conclusion There is cellular immune disorder in chronic hepatitis B. The function of Th2 cell increases when the patients are admitted to hospital. The function of Th2 cell is more powerful when the condition of patients is severer. When the live. function is normal, the function of Th2 cell and the HBV quantity decrease.
出处
《临床消化病杂志》
2006年第6期345-347,共3页
Chinese Journal of Clinical Gastroenterology
