摘要
目的 研究慢性乙肝病毒感染者的免疫状态 ,了解肝功能、病毒负荷量同IFN γ、IL -10、IL -2、IL- 12和IL -18等细胞因子之间的相关性。方法 收集HBeAg阳性ALT异常者 40例 (A组 ) ,HBeAg阳性ALT持续正常者 2 0例 (B组 ) ,HBeAg及HBV DNA阴性ALT正常者 2 0例 (C组 ) ,另外选择 15例各型肝炎病原学标志检测均为阴性的健康体检者为正常对照组 ,采用酶联免疫吸附试验(ELISA)测定血清IFN -γ、IL- 10、IL -2、IL- 12和IL- 18水平。结果 IL -2、IFN- γ、IL- 12在各组间无明显差异 ;HBeAg阳性ALT异常组IL 10明显高于其他组 ,HBeAg阳性ALT正常组IL- 18明显低于HBeAg阳性ALT异常组 ,也低于其他组 ;血清IL- 2在病毒量 10 4～ 6组中的含量较其他各组明显偏高。血清IL -10在≤ 10组较其他组显著偏低。 10 8组中IL -18的含量较其他各组明显偏低。IFN -γ、IL -12在各病毒量组中无显著差异。结论 IL -10和IL -18水平与乙肝病毒感染状态和病毒量具有一定的相关性。
Objective To explore different immune status among various types of chronic hepatitis B and to investigate correlation of hepatic function (serum ALT), HBV DNA,with serum IFN-γ, IL-10, IL-2, IL-12 and IL-18.Methods Eighty patients of various types of chronic hepatitis B were enroll, including 40 HBeAg positive with abnomal ALT, 20 HBeAg positive with persistent nomal ALT, 20 HBeAg and HBV DNA negative with persistent nomal ALT.Results In patients of HBeAg positive with abnomal ALT, serum IL-10 were significantly higher than that in other groups. Serum IL-18 were significantly lower in group of HBeAg positive with nomal ALT than that in group of HBeAg positive with abnomal ALT (P<0.05); Serum IL-2 in HBV DNA 10 4-6 group were a little higher than that in other groups.Serum IL-10 in HBV DNA<103 group were significantly lower than that in other groups.IL-18 were significantly higher in HBV DNA 108 group (P<0.05). Conclusion There are differences in serum IL-10,IL-18 among various types of chronic hepatitis B patients.
出处
《胃肠病学和肝病学杂志》
CAS
2005年第1期82-83,86,共3页
Chinese Journal of Gastroenterology and Hepatology
基金
上海市教委课题 (30 30 4 1 80 9)
