摘要
研究了仙人掌多糖水提法的最佳工艺条件,以及仙人掌多糖的分离纯化。试验结果表明,提取液用Savag法脱蛋白后,采用SephacrylS-400柱层析纯化,得到仙人掌多糖OP,经过高效凝胶渗透色谱和常压凝胶柱色谱分析表明,OP为均一多糖,高效液相凝胶色谱法测定OP分子量(Mw)为172591Da。
The extraction technology, separation and purification of the polysaccharide from opuntia was studied in this paper. By using Savag method, almost all protein was removed from the extracted juice. Then the polysaccharide was purified by Sephacryl S-400 gelcolumn chromatography, and OP was gained. Its homogeneity is verified by HPGPC and Sephadex G-200 column chromatography. And the molecular weight of OP is 172591Da by the determination of HPGPC.
出处
《现代食品科技》
EI
CAS
2006年第2期138-140,149,共4页
Modern Food Science and Technology
关键词
仙人掌多糖
提取
分离纯化
分子量测定
Opuntia
Polysaccharide
Extraction
Isolation
Purification
Determination of molecular weights
