摘要
目的建立2日龄SD大鼠脑室周围白质软化(PVL)动物模型,并进行评价。方法2日龄SD大鼠65只,随机分为PVL组和对照组。PVL组行右侧颈总动脉结扎手术,6%氧、94%氮混合气体处理4 h,分别在缺氧后72 h,14及28 d处死;对照组行假手术,不进行缺氧处理,时间段与PVL组配对。模型评价方法:常规HE染色及电镜检查;免疫组化学观察PVL72 h组胶质纤维酸性蛋白(GFAP)、β淀粉样前体蛋白(-βAPP)、O4和PVL 14 d组髓磷脂碱性蛋白(MBP)改变;采用悬吊、倾斜板、旷场和圆筒试验对术后28 d大鼠进行神经行为学检测。结果HE染色和电镜显示PVL组早期右侧侧脑室周围脑白质损害,远期并脑室扩大,髓鞘减少。免疫组织化学显示PVL组大鼠白质GFAP和-βAPP阳性染色A值较对照组明显增强,GFAP阳性细胞平均直径较对照组增大;PVL组大鼠MBP阳性染色较对照组减弱;O4阳性标记的异常细胞数较对照组增多。神经行为学检测PVL组与对照组织比较有明显异常。结论2日龄SD大鼠右侧颈总动脉结扎-缺氧模型符合PVL病理及行为改变。
Objective To establish the 2 day- old SD rats model of periventticular leukomalacia （PVL）. Methods Sixty - five healthy 2 day- old SD rats were randomly divided into 3 experimental PVL groups and 3 control groups. The experimental PVL groups were subjected to tight carotid ligation（RCL）,and then they were suffered from hypoxia by 6% oxygen and 94% nitrogen for 4 hours. Meanwhile sham surgeries were performed on control groups without exposed to hypoxia. Light and electronic microscopy were used to observe brain pathological changes. Immunohistochemaistry methods were used to detect the distribution and expression of glial fibrillary acidic protein（GFAP） ,β- amyloid precursor protein（β - APP） ,myelin basie protein（MBP） and 04 of brain at 72 hours post - operation. Hanging test, inclined plane test,open field test and cylinder test were performed on the rats 28 days post - operation. Results 1. In the PVL groups, light and electronic microscopy showed that tissue necrosis was observed in the periventricular white matter area at early stage, and at later stage tight ventricular dilation,decrease of the corpus callosum area and loss of medullary sheath were detected. The morphomettical analysis showed that GFAP and β - APP integrated optical density（A） of PVL group was increased, and mean diameter of GFAP - immunoreactive cells was also increased in PVL group, while MBP A of PVL group was decreased compared with contral group. The density of O4 - immunoreactive abnormal cells was dramatically increased in PVL group. The outcomes of neurobehavioral tests of PVL group were greatly abnormal compared with control group.Concluion The changes in 2 - day- old SD rats,RCL - hypoxia model are accorded with the pathology and behavior characeriatis of PVL.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第19期1330-1332,共3页
Journal of Applied Clinical Pediatrics
基金
教育部高等学校博士学科点专项科研基金项目资助(20050610071)
