摘要
目的探讨泡泡浴水疗对新生期反复惊厥大鼠神经行为损伤的干预效应及其分子机制。方法出生6 d(P6)的SD大鼠36只,随机分为对照组(n=12)、惊厥组(n=12)和水疗组(n=12)。惊厥组及水疗组大鼠在P6时吸入三氟乙醚诱导惊厥发作,持续30 min,连续6 d;对照组同样操作,但不吸入三氟乙醚。水疗组于惊厥结束第2天开始进行水疗干预,为期28 d。水疗期间分时段进行负向趋地试验、悬吊试验行为学评分,水疗结束后采用Timm染色观察各组大鼠海马苔藓纤维发芽情况,荧光实时定量(RT)-PCR测定各组大鼠大脑皮质可塑性相关基因(PRG)-1 mRNA、PRG-3 mRNA的表达。结果 1.负向趋地试验评分惊厥组较对照组降低(P<0.05),经泡泡浴水疗,水疗组与对照组无显著差异;悬吊试验各组差异均无统计学意义。2.Timm染色显示,惊厥组大鼠海马齿状回内分子层和CA3区锥体细胞层可见明显异常增生苔藓纤维,水疗组异常增生苔藓纤维密度较惊厥组降低,对照组未见明显发芽。3.RT-PCR显示惊厥组大鼠大脑皮质PRG-1 mRNA、PRG-3 mRNA表达较对照组明显升高,差异均有统计学意义(Pa<0.05)。水疗组大鼠大脑皮质PRG-3 mRNA表达较惊厥组显著降低(P<0.05),PRG-1 mRNA表达虽然亦较惊厥组降低,但其差异无统计学意义(P>0.05)。结论新生期大鼠吸入三氟乙醚致反复惊厥后能够导致神经行为发育损伤,大脑皮质PRG-1、PRG-3参与发育期惊厥性脑损伤的病理生理机制,早期进行泡泡浴水疗干预对神经损伤有修复作用。
Objective To explore the intervention effects and molecular mechanism of bubble bath hydrotherapy on prolonged neonatal seizure induced neurobehavioral damage.Methods Thirty-six neonatal rats were assigned into control group（n=12）,recurrent seizure group（RS group,n=12）,and bubble bath hydrotherapy group（HRS group,n=12）.The volatile agent flurothyl was used to induce 30 min seizure attack.At postnatal day 6（P6）,recurrent seizures were induced once per day for consecutive 6 days in the RS group and HRS group.The rats in control group were placed into the container for an equal amount of time to their counterpart without exposure to flurothyl.In HRS group,hydrotherapy intervention was used after the end of the last seizure for 28 consecutive days.During the hydrotherapy,neurobehavioral changes were analyzed using the negative trend and suspension scores.Mossy fiber sprouting and plasticity related gene（PRG）-1 mRNA,PRG-3 mRNA in cerebral cortex were determined subsequently by Timm staining and real-time（RT）-PCR methods.Results 1.The scores of the negative trend experiment in RS group was significanty lower than that in control group（P0.05）.After hydrotherapy,however,there was no significant difference between control group and HRS group.In addition,there was no significant difference in the test of suspension.2.Timm staining showed that aberrant mossy fiber sprouting was seen in the inner molecular layer of the granule cells of dentate gyrus and the stratum pyramidal of CA3 subfield in RS group.In HRS group,aberrant mossy fiber density was lower than that in the RS group.3.RT-PCR analysis showed that expressions of PRG-1 mRNA and PRG-3 mRNA in cerebral cortex of RS group increased significantly than those in control group（Pa0.05）.The expression of PRG-3 mRNA in HRS group decreased significantly than that in RS group.The expression of PRG-1 mRNA in HRS group also decreased than that in RS group,but the difference wans′t significance（P0.05）.Conclusions Prolonged neonatal seizures c
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2011年第7期520-523,共4页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金(30470555
30870808)
江苏省自然科学基金(BK2007509
BK2010233)
江苏省高校自然科学基金(07KJB320103)
关键词
惊厥
神经行为
发芽
大脑皮质
可塑性相关基因
大鼠
新生
seizure
neurobehavioral
sprouting
cerebral cortex
plasticity related gene
neonatal rat
