摘要
研究了海藻酸钙包埋法固定米根霉在三相流化床反应器中间歇发酵生产Ｌ－乳酸的工艺。实验结果表明，固定化颗粒具有较好的重复使用效果；并考察了颗粒装填密度、通气量、糖浓度及温度对产酸速率的影响，在通气量大于１．０ｖｖｍ、温度为３９℃、糖浓度为（１０～８０）ｇ／Ｌ的条件下，产酸速率达到２０．６ｇ／Ｌ·ｈ，糖对Ｌ－乳酸的转化率为（７０～８０）％；
The L lactic acid production by R. oryzae immobilized in calcium alginate beads in a three phase fluidized bed bioreactor was studied. As a result, the immobilized cells were reused successfully for 6 batch cycles and retained their fermentation activity. The effects of the volume of beads packed in the bioreactor, aeration rate, concentration of glucose in the medium and temperature on fermentation were investigated. When the aeration rate was greater than 1.0 vvm, the concentration of glucose was about 10￣80 g/L and the temperature was 39℃,the production rate reached to 20.6 g/(1 beads·h) and the yield was 70￣80 g L lactic acid/100 g glucose consumed. Mathematical model of concentration of glucose and temperature on fermentation rate was established.
出处
《化学反应工程与工艺》
CAS
CSCD
北大核心
1996年第3期301-307,共7页
Chemical Reaction Engineering and Technology
基金
国家自然科学基金资助
关键词
米根霉
固定化
发酵
流化床反应器
乳酸
L Lactic acid, Rhizopus oryzae, Immobilized cell fermentation, Three phase fluidized bioreactor
