摘要
用聚氨酯泡沫块固定黄孢原毛平革菌（Ｐｈａｎｅｒｏｃｈａｅｔｅｃｈｒｙｓｏｓｐｏｒｉｕｍ），在摇瓶条件下研究了合成木素过氧化物酶的特点和重要影响因素。固定化培养与游离菌丝球培养相比，最高酶活提高６４％。固定化细胞重复利用４批仍保持较高的产酶活性。高剪应力对酶活有抑制作用。苯甲醇可以代替藜芦醇强化木素过氧化物酶的合成，其最佳浓度为５．２ｍＭ。利用自制的三相流化床反应器进行了间歇产酶试验，发现最佳通气量为１．０ｖｖｍ，最高酶活可达３８０Ｕ．Ｌ－１，比摇瓶高一倍。
Lignin peroxidase(Lip) production by Phanerochaete chrysosporium immobilized onpolyurethane foams was studied in shake flasks first. The maximum Lip activity in the immobilizedcell culture was 64% higher than that in the free pellets culture. The immobilized-cell was stable forLip production during 4 repeated batch cultures. High shear stress inhibited Lip production. Benzylalcohol could enhance the Lip production similar to veratryl alcohol with optimal concentration of5.2mM. In a three-phase fluidized-bed reactor, the maximum Lip activity in the batch culture was380U. L-1, with the optimal air flux of 1.0 vvm, which was twice higher than that in the shake flaskculture.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
1999年第2期135-140,共6页
Journal of Chemical Engineering of Chinese Universities
基金
浙江省自然科学基金
关键词
木素过氧化物酶
黄孢原毛平革菌
固定化
合成
Lignin peroxidase, Phanerochaete chrysosporium, Immobilization, Three-phasefluidized bed
