摘要
目的:通过扭体法、热板法和电刺激法观察迎春花提取物对小鼠的镇痛作用及对小鼠的镇静作用。方法:实验于2003-04/06在赣南医学院药理教研室完成。①小鼠50只,随机数字表法分为5组,每组10只,分别为生理盐水组(腹腔注射20mL/kg),迎春花提取物5,10,20g/kg组,吗啡0.01g/kg组。给药后30min,各组小鼠均腹腔注射6g/L醋酸0.2ml/只,观察20min内小鼠扭体次数。②取40只雌性小鼠,随机数字表法分为4组,每组10只,分别为生理盐水组,迎春花提取物10,20g/kg组,氨基比林0.2g/kg组。用热板法测定小鼠给药后15,30,60min痛阈值。③小鼠40只,随机数字表法分为4组,每组10只,分别为生理盐水组,迎春花提取物5,10g/kg组,吗啡0.01g/kg组。用电刺激法测定给药后30,45,60min镇痛率。④小鼠40只,随机数字表法分为4组,每组10只,将小鼠放入吊笼描记波形10min后分别将小鼠腹腔注射生理盐水20ml/kg,迎春花提取物5,10g/kg,地西泮0.005g/kg。30min后放回吊笼,记录用药后活动波形5min。⑤小鼠40只,随机数字表法分为4组,每组10只,分别为生理盐水组,迎春花提取物10,20g/kg组,地西泮0.005g/kg组,给药后30min,再腹腔注射戊巴比妥钠50mg/kg,记录小鼠睡眠持续时间。结果:纳入小鼠210只,均进入结果分析。①扭体实验中,迎春花提取物5,10,20g/kg组、吗啡组扭体次数明显低于生理盐水组,差异有显著性意义[分别为(3.2±3.1),0,(1.9±3.5),0,(84.7±15.1)次,P<0.001]。②热板法实验中,迎春花提取物10,20g/kg组、氨基比林组给药后15,30min痛阈值均高于生理盐水组,差异有显著性意义[分别为(43.5±12.1),(57.2±8.9),(60.0±0.0),(28.2±3.4)s;(44.7±11.2),(60.0±0.0),(60.0±0.0),(27.9±4.3)s,P<0.01];迎春花提取物20g/kg组、氨基比林组给药后60min痛阈值均高于生理盐水组,差异有显著性意义[分别为(60.0±0.0),(56.9±4.9),(26.4±3.8)s,P<0.01],迎春花提取物10g/kg组与生理盐水组比较差异无显著性意�
AIM： To observe the analgesic effect and sedation effect of jasminum nudiflorura lind （JNL） extract in mice through writhing body test, hot plate and electric stimulation. METHODS： The experiments were conducted in the Department of Pharmacology, Gannan Medical College from April to June 2003. ①Fifty mice were randomly divided into five groups with 10 in each group： saline group intraperitoneal injection 20 mL/kg, 5, 10, 20 g/kg JNL extract groups and 0.01 g/kg morphine group. After 30 minutes, each mouse was administrated with 0.2 mL 6 g/L glacial acetic acid through intraperitoneal injection, and the times of writhing body was recorded within 20 minutes. ②Forty female mice were randomly divided into saline group, 10 g/kg, 20 g/kg JNL extract groups and 0.2 g/kg amidopyrine group with 10 rats in each. The pain threshold value was determined by hot plate test at 15, 30 and 60 minutes after administration. ③ Forty qualified mice were randomly divided into saline group, 5 g/kg, 10 g/kg JNL extract groups, 0.01 g/kg morphine group with 10 rats in each. The rate of analgesia was determined by electric stimulation at minutes 30, 45 and 60 after administration.④Forty mice were randomly divided into four groups with 10 rats in each. Each mouse was put in suspension cage and wave shape was recorded in 10 minutes, whereafter were treated with 20 mL/kg saline, 5 g/kg, 10 g/kg JNL extract and 0.005 g/kg diazepam through intraperitoneal injection. After 30 minutes each mouse was put in suspension cage again and wave shape was recorded for 5 minutes. ⑤Forty mice were randomly divided into saline group, JNL groups of 10 g/kg, 20 g/kg and diazepam group of 0.005 g/kg. Thirty minutes after administration, they were given sodium pentobarbital of 50 mg/kg through intraperitoneal injection, eventually sleeping duration of mice were recorded. RESULTS： Totally 210 included mice entered the result analysis. ①In the writhing body test, the times of writhing body in the 5, 10 and 20 g/kg JNL extract groups and th
出处
《中国临床康复》
CSCD
北大核心
2006年第35期42-44,共3页
Chinese Journal of Clinical Rehabilitation
