摘要
背景:夜来香水提取物具有抗心律失常、局部麻醉以及中枢抑制作用。目的:探讨夜来香提取物对小鼠的镇痛作用,为临床疼痛治疗寻找新药。设计:随机对照观察。单位:赣南医学院现代教育中心与药理教研室。材料:实验于2005-03/04在赣南医学院科研中心实验室完成。①选取健康成年昆明种小鼠150只用于以下4个独立实验。②药品:夜来香提取物由沈阳药科大学植化教研室提供(药品批号:2002080901);盐酸吗啡注射液(沈阳第一制药厂,批号000305);盐酸纳洛酮注射液眼盐侨(湖南)制药有限公司,批号20021109演。方法:①夜来香提取物对醋酸引起小鼠扭体反应的实验:小鼠40只,随机数字表法分为4组,10只/组,分别腹腔注射0.02mL/g生理盐水,0.10,0.20mg/g夜来香提取物,0.10mg/g氨基比林。15min后腹腔注射6g/L冰醋酸0.01mL/g,观察记录15min内各组小鼠扭体反应次数。②夜来香提取物对小鼠热板法致痛作用的实验:雌性小鼠40只,随机数字表法分为4组,10只/组,分别腹腔注射0.02mL/g生理盐水,0.10,0.20mg/g夜来香提取物,0.01mg/g吗啡,用热板法测定给药后15,30,60min的痛觉反应。③纳洛酮拮抗吗啡、夜来香提取物对小鼠热板法致痛作用的实验:雌性小鼠30只,随机数字表法分为3组,10只/组,分别腹腔注射0.02mL/g生理盐水,0.004mg/g纳洛酮+0.01mg/g吗啡,0.004mg/g纳洛酮+0.10mg/g夜来香提取物,热板法测定给药后15,30,60min的痛觉反应。④夜来香提取物对电刺激致痛的实验:小鼠40只,随机数字表法分为4组,10只/组,分别腹腔注射0.02mL/g生理盐水,0.10,0.20mg/g夜来香提取物,1g/L吗啡,于给药后20,35,50,70min重复电刺激,电刺激法测定痛觉反应。主要观察指标:①小鼠扭体反应次数。②热板法致小鼠痛觉反应的时间。③电刺激法致小鼠镇痛率。结果:共选取健康成年昆明种小鼠150只用于4个独立实验,全部进入结果分析。①小鼠扭体反应次
BACKGROUND： It has been considered that Cestrum noctumum L. （CNL） has the effects of antiarrhythmia, local anesthesia and central inhibition. OBJECTIVE： To investigate the analgesic effect of CNL extract on mice, so as to find new drugs for clinical treatment of pain. DESIGN： A randomized control observation. SETTING： Center of Modem Education and Department of Pharmacology, Gannan Medical College. MATERIALS： The experiments were carried out in the laboratory of scientific research center, Gannan Medical College between March and April in 2005.①A total of 150 healthy adult Kunming mice were used in four independent experiments. ②Drags： CNL extract was provided by the Department of Phytochemistry, Shenyang Pharmaceutical University （batch number： 2002080901）, morphine hydrochloride injection by Shenyang No.l Pharmaceutical Factory （batch number： 000305）, and naloxone hydrochloride injection by Yanqiao （Hunan） Pharmaceutical Co. Ltd.. （batch number： 20021109）. METHODS： ① Effects of CNL extract on writhing times induced by acetic acid： Forty female mice were randomly divided, into four groups with 10 mice in each, and they were treated with intraperitoneal injections of 0.02 mL/g saline, 0.10 and 0.20 mg/g CNL extract and 0.10 mg/g aminophenazone respectively. The intraperineal injection of 6 g/L glacial acetic acid was given after 15 minutes. The writhing times of mice within 15 minutes were observed and recorded in each group. ② Effects of CNL extract on the pain induced by hot pla in mice： Forty female mice were randomly divided into four groups with 10 mice in each, and they were treated with intraperineal injections of 0.02 mL/g saline, 0.10 and 0,20 mg/g CNL extract and 0,10 mg/g morphine respectively. The pain responses were detected at 15, 30 and 60 minutes after administration, ③ The antagonistic effect of naloxone on morphine and CNL extract to the pain induced by hot plate in mice： Thirty female mice were randomly divided into three groups with 10 mi
出处
《中国临床康复》
CSCD
北大核心
2006年第35期172-174,共3页
Chinese Journal of Clinical Rehabilitation
