摘要
目的观察乙型肝炎病毒对慢性乙型肝炎患者NK细胞亚群的影响。方法利用流式细胞术检测20例正常人及62例慢性乙型肝炎患者人外周血淋巴细胞CD3、CD4、CD8、CD56、CD16的表达;同时观察NK细胞亚群改变与乙型肝炎病毒携带量的相关性。结果①人类NK细胞根据CD56分子的表面密度可分为两个截然不同的群体,绝大多数(约90%)的NK细胞低水平表达CD56(CD56dim)且高表达CD16,少数NK细胞(10%)高水平表达CD56(CD56bright)且缺乏或低水平表达CD16;②慢性乙型肝炎患者CD56brightNK细胞的表达及绝对数量较正常对照组无明显的改变,CD3-CD56+、CD3-CD56dimNK细胞的绝对数量较正常对照组显著减少(P<0.05);③慢性乙型肝炎患者NK细胞亚群改变与患者的病毒携带量无关;④慢性乙型肝炎患者CD3+、CD3+CD4+比例及绝对数量较正常对照组显著减少(P<0.05)。结论机体感染乙型肝炎病毒后,导致CD56dim增殖的关键因子IL-2、IL-21活性下降,使CD56dim数量选择性减少,减弱NK细胞介导机体ADCC的功能。
Objective To evaluate the effect of HBV on NK cell subsets of chronic HBV patient （CHB） patients. Methods Samples from 62 CHB patients and 20 healthy controls were analyzed by immunofluorescence and flow cytometry to determine the expression of CD3, CD4, CD8, CD56, and CD16 on lymphocyte. At the same time, the relationship between the change of NK subsets and the number of HBV were investigated. Results ① There were two distinct subsets of human NK cells according to cell surface density of CD56. The majority （approximately 90%） of human NK cells were CD56^dim and express high levels of CD16, whereas minority （approximately 10%） were CD56^bright and CD16dim/neg. ② In this study, the analysis of CD56^dim and CD56^bright NK subsets showed that neither the number nor the phenotype of CD56^bright NK cells were significantly altered in CHB patient, whereas the number of CD56^dim NK cells was decreased. ③ There was no relationship between the change of NK cell subsets and the number of HBV. ④ As compared with controls, the number and phenotype of CD3^＋ and CD3^＋ CD4^＋ T cells in CHB patients were significantly decreased （P 〈 0.05）. Conclusion After infected with HBV, the activity of IL-2 and IL-21 which could induce CD56^dim proliferation is decreased, thus the number of CD56^dim NK cells selectively decreased and the function of ADCC induced by NK cells is reduced.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2006年第5期559-561,共3页
Immunological Journal
