摘要
目的 NK细胞在抗病毒免疫过程中发挥了重要作用,本研究观察蒙族慢性HBV感染免疫耐受期和免疫清除期患者外周血NK细胞的表型和功能特点,并密切结合患者的临床指标进行分析,以期阐明抗病毒的作用机制。方法采集免疫耐受期患者24例、免疫清除期患者21例及健康对照者25例,分离外周血单个核细胞,采用流式细胞仪测定外周血NK细胞的表型、功能,及临床指标(ALT和病毒载量)。结果与免疫耐受期患者和健康对照组比较,免疫清除期患者外周血NK细胞亚群发生显著改变,表现为CD56dimNK亚群明显下降,而CD56bright NK亚群明显上升;慢性乙型肝炎免疫清除期患者的外周血NK细胞表面的活化标志物如HLA-DR,CD38和CD69的表达显著上升,差异具有统计学意义(P<0.05)。结论蒙族慢性乙型肝炎免疫清除期患者外周血NK细胞处于高活化状态,进一步表明慢性乙型肝炎患者NK细胞的高度活化与肝损伤密切相关。
Objective To investigate phenotype and function of NK cells in patients with chronic hepatitis B virus infection in Mongolian,to analyze the clinical parameters（ALT and viral load）correlated to these immunological features,to study the immune-pathological mechanisms of anti-viral therapy.Methods Peripheral blood NK cells were obtained from24 immune-tolerant carriers,21 immune-activated patients and 25 healthy controls.Peripheral blood mononuclear cells were collected.The phenotype and function of NK cell and clinical parameters（ALT and viral load）were examined by flow cytometry.Results We found that redistribution of peripheral chronic hepatitis B NK cells subsets in immune-activated was significantly different compared with immune-tolerant carriers and healthy control,whose CD56 bright NK cells was significantly increased,CD56 dim NK cells was significantly decreased.NK cells expressing NK activation receptors such as HLA-DR,CD38 and CD69 preferentially accumulated in immune-activated patient,in comparison with those of immune-tolerant carriers and healthy subjects.Conclusions In immune-activated patients,hepatic NK cells are activated and preferentially toward cytolyic activity,which potential correlates with liver injury in Mongolian.
出处
《中国煤炭工业医学杂志》
2013年第7期1073-1077,共5页
Chinese Journal of Coal Industry Medicine
