摘要
目的探讨封闭抗体免疫疗法对免疫性不孕症与习惯性流产病人治疗的效果。方法对138例不孕症及习惯性流产病人行封闭抗体免疫治疗2个疗程。采用单向混合淋巴反应封闭试验、单向混合淋巴细胞反应封闭抑制试验、细胞毒试验及封闭抗体流式细胞分析术检测治疗前后病人混合淋巴细胞反应封闭效率（MLR—BE）、抗独特型抗体、细胞毒指数（CI）及抗CD抗原的封闭效率（抗CD-BE）。结果治疗后不孕症及习惯性流产病人的MLR—BE、抗独特型抗体、CI和抗CD-BE均有不同程度的上升，与治疗前相比，差异均有显著性（t=2．32～38．66，P〈0．05、0．01）。治疗2疗程后与治疗1疗程后相比，差异亦有显著性（t=2．15～12．46，P〈0．05、0．01）。结论封闭抗体免疫疗法治疗免疫性不孕症及习惯性流产具有重要的临床意义和应用价值。
Objective To evaluate the clinical effects of immunosuppressive treatment using blocking antibodies on immune infertility and habitual abortion. Methods This study was conducted in 138 patients who were given blocking antibodies for two courses. One-way mixed lymphocyte reaction blocking test and complement-dependent cytotoxic test, anti-idiotypic antibodies to blocking antibodies by one-way mixed lymphocyte reaction blocking inhibiting test were used to monitor blocking antibodies, and flow cytometry evaluation of blocking antibodies was used to investigate effect of blocking antibodies on the CD antigens of T cells. T-Test was used for statistical analysis. Results After one course of treatment, the levels of MLR-BE, their anti-idiotypic antibodies, CI, anti-CD3-BE, anti-CD4-BE, anti-CD8-BE, and anti-CD4-BE/anti-CD8-BE of immune infertility greatly increased compared with those of before treatment （t=2.32-38.66;P〈0.05, 0.01）. After two courses, the MLR-BE, the antiidiotypic antibodies, CI, anti-CD3-BE, anti-CD4-BE, anti-CD8-BE, and anti-CD4-BE/anti-CD8-BE of immune infertility greatly increased compared with those of before treatment, and the difference was significant compared with those after one course of treatment （t=2.15-12.46; P〈0.05, 0.01）. Conclusion The immunosuppressive therapy using blocking antibodies is of great clinical significance and value for infertility and habitual abortion.
出处
《齐鲁医学杂志》
2006年第5期401-403,共3页
Medical Journal of Qilu
关键词
不孕症
免疫
流产
习惯性
抗体
封闭
疗法
免疫
infertility, immune
abortion, habitual
antibodies, blocking
therapy, immunosuppressive
