摘要
目的 :探讨人类白细胞抗原 (humanleukocyteantigen ,HLA)、封闭抗体 (blockingantibody,BA)两种免疫因素与妊高征的关系。方法 :采用顺序特异性引物聚合酶链反应技术 (PCR SSP)测定妊高征组及对照组各 38例HLA DR4抗原基因 ,应用流式细胞仪检测封闭抗体对配偶CD3、CD4、CD8抗原的封闭作用。结果 :妊高征患者HLA DR4抗原基因检出率 (36 .84 % )高于对照组 (15 .79% ) ,差异有显著性 (P <0 .0 5 ) ;重度妊高征夫 -妻和母 -胎间HLA DR4相容性增加 ,差异有显著性 (P <0 .0 5 ) ;妊高征组封闭抗体对配偶CD3、CD4、CD8抗原的封闭作用明显低于正常妊娠组 ,差异有显著性 (P <0 .0 5 )。结论 :母胎间免疫失衡可能与妊高征发病有关。
Objective:To study the relation between human leukocyte antigen(HLA), blocking antibody(BA) with pregnancy induced hypertension(PIH). Methods: using polymerase chain reaction with sequence specific primers(PCR-SSP),the HLA-DR4 were detected in PIH families and normotension families. The maternal serum blocking effect on paternal antigens CD3,CD4 and CD8 were detected by flow cytometry. Results: The HLA-DR4 positive rate of PIH women (36.84%)was higher than that of normal women(15.79%). The HLA-DR4 antigen sharing in couples and feto-maternal in severe PIH were increased. In women with PIH the blocking effect on paternal antigens CD3、CD4 and CD8 was significantly lower than in normal pregnancy. There was statistical difference between the two groups(P<0.05). Conclusions: The maternal-fetal imbalance of immune factors may be involved in the pathogenesis of PIH.
出处
《广州医学院学报》
2004年第3期34-36,共3页
Academic Journal of Guangzhou Medical College
关键词
妊娠高血压综合征
免疫
人类白细胞抗原
抗体
pregnancy induced hypertension
Immunization
human leukocyte antigen
antibody
