摘要
代谢工程是用现代基因工程技术对细胞性质进行改进.代谢工程包括3个重要步骤:细胞途径的修饰(合成),修饰后细胞表型的严格评价(表型表征),根据评价结果设计进一步的修饰(优化设计).其中“组学”技术(基因组学、转录物组学、蛋白质组学、代谢物组学、代谢通量组学等)在表型表征中起着极为重要的作用.此外,由于细胞的性质不仅与其基因组密切相关,而且也与细胞所处的微观及宏观环境条件(底物种类与浓度、pH、温度、溶氧、生物反应器的混合与传递特性等)密切相关,因此在菌种改进过程中就要密切结合这些实际的环境条件对所得的菌株用上述“组学”技术进行表型表征,从而使所开发的代谢工程菌株满足包括环境条件影响在内的整个生物加工过程优化的需要.本文结合国际上的发展及作者的研究实践,综述了近几年基于功能基因组学的代谢工程研究发展情况.
Metabolic engineering is the improvement of cellular properties by using modern genetic engineering tools. Metabolic engineering encompasses three important steps： the modification of cellular pathways （synthesis）, the rigorous evaluation of the phenotype of the modified cells （phenotype characterization） and optimization design of further genetic modification （design）. In the procedure, ＂Omics＂ （genomics, transcriptomics, proteornics, metabolomics, fluxomics） analyses play a very important role in the phenotype characterization. Because the environmental conditions have also great effects on the cellular properties, so the changes of the cellular properties under different environmental conditions must be evaluated by the ＂Omics＂ analyses. Based on the ＂Omics＂ analyses, it is possible to get valuable knowledge that can be used for the development of metabolic engineering strategies, which consequently are used to optimize the bioprocess. In this paper, the recent advances of metabolic engineering combined with functional genomics are reviewed.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2006年第8期1792-1801,共10页
CIESC Journal
基金
国家自然科学基金项目(20536040)
国家重点基础研究发展计划项目(2003CB716003)
天津市科技发展项目(05YFGZGX04500)
教育部长江学者和创新团队发展计划项目.
