摘要
猪带绦虫45W基因已被认为是预防猪囊虫病的基因工程疫苗候选基因之一。其中4B基因是45W基因家族中高度保守的一个成员(以下称45W-4B)。本试验将重组于pGEM-3Z载体上的4B基因转载到pVL1393转移载体中,通过鉴定获得重组质粒pVL1393-4B,然后将重组质粒与BmNPV病毒共转染,筛选出重组BmNPV-4B重组病毒,用该重组病毒感染Bm细胞,收集细胞上清,SDS-PAGE电泳鉴定表达蛋白,并经Westernblot检测表明该蛋白能识别囊虫病人(猪)阳性血清,45W-4B蛋白的成功表达对进一步研究45W蛋白的结构和功能以及开发高效猪囊虫基因工程疫苗打下了基础。
Expression system of BmNPV-Bm is high effective for producing recombinant protein approach to its nature structure by the correct post-transcript process and modification. 45W-4B gene of Taenia solium was cloned into baculovirus transfer vector of pVL1393 and cotransfected into Bomby-x mort nuclear polyhedrosis virus （BmNPV）. The recombinant BmNPV was screened, and used to infect BmN cells and silkworm. 4B protein of expression was detected in the cell supernatant and haemolyph by Western blot. Successful expression of 4B recombinant protein in BmNPV-Bm will be significant for study of the structure and function of 4B protein, and development of the effective recombinant vaccine against T.solium and Cysticercus cellulosae.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2006年第5期527-530,共4页
Chinese Journal of Preventive Veterinary Medicine
