摘要
目的观察猪带绦虫混合重组双歧杆菌（Bh）疫苗免疫仔猪后诱导产生的保护效果。方法将40d龄健康仔猪分为实验组和对照组，每组4头。实验组以10“菌落形成单位（CFU）重组Bb-TS045W-4B疫苗和重组Bb—TSOL18疫苗，按1：1混合后灌胃免疫，间隔2周免疫1次，共免疫2次；对照组以Bb液体培养基（MRS）50ml灌胃。在免疫后不同时间点（O、2、4、6、8周）取前腔静脉血，分离血清，采用酶联免疫吸附试验（ELISA）法检测血清IgG水平。末次免疫4周后用猪带绦虫卵进行攻击，感染3个月后剖杀仔猪，分离囊尾蚴，计算减蚴率；取前腔静脉血，分离血清和制备外周血淋巴细胞（PBMC），采用ELISA法检测血清IgG、IgG1、IgG2a水平和PBMC培养上清液白细胞介素（IL）-2、干扰素（IFN）-1、IL．4及IL-10水平；四甲基偶氮唑盐（MTT）法检测PBMC增殖水平。结果实验组血清IgG水平在首次免疫后2～8周升高，在4周达较高水平，与对照组比较，差异有统计学意义（mg／L：270．64±1．94比207．74±2．24，t=42．479，P〈0．05）。攻击感染3个月后，实验组减蚴率为80．48％；实验组与对照组比较，血清IgG、IgG2a水平显著升高，IgGl水平显著降低（mg／L：364．15±11．52比245．94±8．81．89．74±1．13比62．61±0．84，20．52±1．00比34．11±0．65，t=16．303、38．579、-22．772，P均〈0．05）；PBMC培养上清液IL-2、IFN-叫水平显著升高，IL-4、IL-10水平显著降低（ng／L：215．24±3．31比174．19±2．14．28．21±0．27比17．69±0．28．40．35±0．34比52．57±0．29．71．34±0．36比94．82±0．45，t=20．839、53．623、-54．743、-81．266，P均〈0．05）；PBMC增殖水平显著升高（0．620±0．051比0．242±0．053，t=10．259，P〈0．05）。结论猪带绦虫混合重组Bb疫苗可诱导仔猪产生-定的保护力，Th1型免疫应答在保护性免疫机制中起重要作用。
Objective To study the protective effect of a mixed recombinant Bifidobacterium （Bb） vaccine of Taenia solium in piglets. Methods Healthy piglets of 40 days old were randomly divided into experimental group and control group, 4 in each group. Experimental group was given 10H CFU rBb-TSO45W-4B vaccine and rBb-TSOL18 vaccine （in the ratio of 1 ： 1）. Control group was given Bb liquid medium （MRS）. A total of two times of immunization were conducted, once for every two weeks. At different time points after immunization, the serum was separated from precaval vein blood to detect the level of IgG by enzyme linked immunosorbent assay （ELISA）. Piglets were challenged with Taenia solium eggs on the 4th week after the last immunization and killed 3 months after infection. The cysticerci were separated to count and calculate the reduction rate of cysticerci. Blood from precaval vein was collected to separate serum and prepare peripheral blood lymphocytes （PBMC）. The levels of IgG,IgG1 and IgG2a in serum samples and the levels of interleukin （IL）-2, interferon （IFN）-γ, IL-4 and IL-10 in PBMC culture supernatant were determined by ELISA. The level of PBMC proliferation was tested using methyltetrazolium （MTT） assay. Results In experimental group, the level of serum IgG increased from the 2nd to the 8th weeks after immunization, and reached the highest level on the 4th week after immunization （mg/L： 270.64 ± 1.94 vs 207.74 ±2.24, t = 42.479, P 〈 0.05）. Reduction rate of cysticercus was 80.48%. Compared with control group, the levels of IgG and IgG2a in serum were significantly increased, while the level of IgG1 was significantly decreased （mg/L： 364.15 ± 11.52 vs 245.94 ± 8.81, 89.74 ± 1.13 vs 62.61 ± 0.84, 20.52 ±1.00 vs 34.11 ±0.65, t = 16.303, 38.579, - 22.772, P 〈 0.05）. The levels of IL-2 and IFN- γin PBMC culture supernatant were significantly increased, while the levels of IL-4 and IL-10 were significantly decreased （ng/L： 215.24± 3.31 vs 174.19 ±2.14, 28
作者
周必英
刘美辰
周泠
杨凤娇
贾启
江楠
王灵军
Zhou BY Liu MC Yang FJ Jia Q Jiang N Wang L J Zhou L(Department of Parasitology, Zunyi Medical College, Zunyi 563000, China Department of Traditional Chinese Medicine and Anorectum, the Affiliated Hospital of Zunyi Medical College, Zunyi 563000, China)
出处
《中华地方病学杂志》
CSCD
北大核心
2017年第8期552-556,共5页
Chinese Journal of Endemiology
基金
基金项目：国家自然科学基金（81160206）
贵州省科技厅科学技术基金项目和遵义市15851人才精英工程资助项目（黔科合J字[2014]2178号）
