摘要
采用反相高效液相色谱法（RP-HPLC），以Kromasil C18柱（250×4．6mm，5μm）为色谱柱，甲醇-0．05M磷酸二氢钾（25：75）为流动相，在254nm检测波长，1．0ml／min流速的条件下建立中风康复片中士的宁的含量测定方法。结果表明，中风康复片中士的宁与其它成分分离良好，士的宁在0．072～0．648μg范围内呈良好的线性关系（r=0．9999），测量方法的平均回收率为95．51％，RSD=0．7％（n=6）。该方法灵敏度高、操作简单、结果准确，可用于本制剂的质量控制。
To establish a RP-HPLC method for determination of strychnine in Zhongfeng Kangfu Tablet. The sample were analyzed by RP-HPLC using Kromasil （250×4.6mm, 5μm） column; methanol -0.05M potassium dihydrogen phosphate （25 ： 75） as mobile phase;UV detector at 245nm and the flow rate was at 1.0ml/min. The result was the content of strychnine had a good linearity in the range of 0. 072～0. 648（μg）,the correlation was 0. 9999,the Average recovery was 95.51%, RSD = 0. 7% （n =6）. The method was sensitive, simple, accurate and suitable for quality control of strychnine in Zhongfeng Kangfu tablet.
出处
《广西科学》
CAS
2006年第3期219-221,共3页
Guangxi Sciences
