摘要
目的:建立复方风湿丸中士的宁和马钱子碱含量测定的高效液相色谱法.方法:色谱柱Diamonsil C18(4.6 mm×200 mm,5 μm);流动相为:甲醇-乙腈-水-冰-醋酸(45:30:25:0.5);检测波长254 nm;流速1.0 ml/min.用硅胶固相萃取的样品预处理方法提取复方风湿丸中的士的宁和马钱子碱.结果:士的宁浓度在10～100 μg/ml,马钱子碱浓度在6～60 μg/ml范围内线性关系良好.士的宁r=0.999 5,平均回收率99.55%,RSD为1.01%;马钱子碱r=0.999 7,平均回收率99.73%,RSD为1.05%.结论:该方法检测结果准确,重现性好,能有效地控制复方风湿丸的质量.
Objective：To establish the H PLC method for detemination of strychnine and brucine in compound Fengshi Pills. Methods; HPLC was performed on Diamonsil C18 （4.6 mm×200 mm,5μm）with methanol-acetonitrile-HAC（45 ： 30 25 ： 0.5）as mobile phase. Detecting wavelength was 254 nm and flow rate was 1.0 ml/min. Strychnine and Brucine in compound Fengshi Pills were extracted by silica gel solid-phase extraction method. Results： Linear ranges of Strychnine and Brucine were 10-100μg/ml and 6μg/ml- 60μg/ml respectively. The recovery rates of strychnine and brucine were 99.55 % （RSD= 1.01 % ）and 99.73 % （RSD= 1.05% ） respectively. Conclusion ： This method is accurate, repeatable and effective for quality control of compound Fengshi Pills.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2005年第9期859-860,共2页
Lishizhen Medicine and Materia Medica Research
