摘要
黑曲霉（Ａｓｐｅｒｇｉｌｌｕｓｎｉｇｅｒ）Ｓ２１经波长２６６ｕｍ，照射剂量８ｍｊ的激光处理４０秒，获得一高产果胶酶突变株ＳＭ４０－６。以果胶为底物培养，该突变株发酵滤液，初筛酶活力达８５４μ／ｍｌ；比出发菌及对照提高２倍。液体发酵工艺条件研究表明，该菌产酶最适培养基组成为甘薯与麦比１∶４，硫胺２％。最适培养条件为：ｐＨ３．５，温度３０℃，培养时间８４小时。
A mutant strain SM40-6 was obtained from Aspergillus niger S21 by treatment with 8 mj of laser irradiation at 266um for 40 seconds. Enzyme activity of culture filltrate of the mutant strain was 854μ/ml, which was about 2 times of its parent strain. The optimal medium consists of sweet potato and wheat bran (1: 4). and 2% ammonium sulfate. The optimal culture conditions are as follows: initial pH3. 5, temperature 30℃ and cultivation time 84 hr.
出处
《西南农业学报》
CSCD
1996年第4期63-68,共6页
Southwest China Journal of Agricultural Sciences
关键词
果胶酶
黑曲霉
发酵
诱变
Mutaant Pectinase Aspergillus niger
Fermentation.
