摘要
基于固定化酶的化学发光停流法测定Ｄ－氨基酸封满良，黄玉文，宫志龙，章竹君（陕西师范大学化学系，西安，７１００６２）关键词固定化酶，化学发光，甲壳质，Ｄ－氨基酸自从发现人体有关组织及血桨中Ｄ－氨基酸的水平与某些疾病有关以来，已报道了许多测定Ｄ－氨基酸的...
D-amino acid oxidase and HRP were separately immobilized on chitosan adsorbed on the silica gels to develop a chemiluminescent method,which was combined with two oxidation reactions of D-amino acid producing H2O2 catalyzed by D-amino acid oxidase and of Luminol-H2O2 generating CL catalyzed by HRP together,also was stopped──flow technique used in this system. It has a good selectivity and sensitivity. Under optimized conditions,the detection limit is 1.8 ×10￣(-5)g/mL,the liner range of D-amino acid concentration was from 8× 10-8to 1. 4×10￣(-6)g/mL,It was practicable to determine D-amino acid in serum directly with satisfactory results.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1996年第12期1859-1861,共3页
Chemical Journal of Chinese Universities
