摘要
目的观察大鼠肾脏缺血-再灌注损伤(IRI)的不同时间内肾组织和血液中IL-6水平的动态变化以及山莨菪碱(654-2)干预的影响。方法构建大鼠肾IRI模型:切除大鼠右肾,夹闭左肾动脉1h,松开再灌注1、4、24h;654-2分别于缺血和再灌注前10min腹腔注射,12h追加一次。用双抗体夹心ELISA法分别动态检测血清和肾组织中IL-6含量,同时做肾功能指标——血尿素氮(BUN)、血肌酐(Scr)和肾系数(RC)检测。结果缺血组IL-6含量低于对照组(P<0.05),而再灌注1h(R1h)组显著高于缺血组(P<0.01)。再灌注4h组(R4h)和24h组(R24h)与缺血组比无明显差异,两组肾组织中IL-6的含量均低于再灌注1h组(P<0.05)。肾组织中IL-6含量的动态变化和血液中IL-6含量的变化呈直线正相关(r=0.86,P<0.01)。654-2干预可使R4h和R24h组的IL-6含量较未处理组增高(P<0.01),同时使R4h组SUN、Scr及肾系数也明显增高(P<0.01或P<0.05),使R24h组的Scr明显降低(P<0.01),但SUN及肾系数无明显变化。结论在再灌注的早期,肾组织和血液中IL-6的含量升高,随之下降接近于单纯缺血水平。654-2预处置使IL-6增多的同时加重早期肾IRI,再灌注24h后才显示其治疗作用。
[Objective] To observe the change of interleukin-6（IL-6） in kidney and blood during renal ischemic repeffusion injury （/RI） in rats and the effect of anisodamine （654-2） intervention. [Methods] The right kidney was reseeted, and the left renal artery was closured by clip for 1 hour and then was loosened to be repeffused for 1 hour, 4 hours and 24 hours. 654-2 was given by peritoneal injection at the time of 10 minutes before ischemia and reperfusion in Group R4h and R24h respectively. In Group R24h, 654-2 was injected again after 12 hours. IL-6 contents in kidney and serum were detected by double antibody sandwich enzyme-linked immunosorbent assay （ELISA）. Serum Urea nitrogen （SUN） and creatinine （Ser） and renal coefficient （RC） were also detected. [Results] IL-6 content in isehemic group was lower than that in sham control group （P 〈0.05）, while in the group of reperfusion for 1 hour （Rlh） was notably higher than that in ischemic group （P 〈0.01）. In the group of repeffusion for 4 hours （R4h） and reperfusion for 24 hours （R24h） , IL-6 contents were not significantly different, but beth in kidney were obviously lower than that in group Rlh （P 〈0.05）. The changes of IL-6 between kidney and serum were in direct correlation in straight line in different groups （r=0.86, P 〈0.01）. With 654-2 preconditioning, IL-6 contents in group R4h and R24h were higher than that in no preconditioning group （NP） （P 〈 0.01）; meanwhile SUN, Scr as well as RC in group R4h were also notably higher than those in NP （P 〈0.01 or P 〈0.05） ; but in group R24h＋654-2, Ser was remarkably lower （P 〈0.01）, while SUN and RC were not different compared with NP group. [Conclusion] IL-6 contents in kidney and blood increase in early stage of reperfusion, then drop off near to the level of ischemic group. 654-2 preconditioning can elevate IL-6 content and aggravate renal injury in early stage of IRI in rats. Up to R24h, 654-2 shows up its cure effect.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2006年第13期1926-1928,1936,共4页
China Journal of Modern Medicine
