摘要
目的探讨丁苯酞对SD大鼠肾脏缺血/再灌注损伤的保护作用。方法将60只SD大鼠采用随机排列表分组法分为对照组、预处理组(单纯丁苯酞灌胃)、假手术组、预处理手术组(丁苯酞灌胃并双肾肾动脉夹闭手术)、手术组(单行双肾肾动脉夹闭手术),每组12只。预处理组和及预处理手术组造模前给予丁苯酞80 mg/(kg·d)灌胃2周,制备SD大鼠肾脏缺血/再灌注急性肾损伤(AKI)模型。检测各组大鼠在缺血/再灌注24 h时血肌酐、尿素氮变化情况,苏木精-伊红染色观察肾脏病理。结果假手术组、预处理手术组和手术组尿素氮水平分别为(8.9±1.6)、(33.2±7.7)、(44.9±17.3)mmol/L,显著高于对照组的(6.1±1.2)mmol/L,差异有统计学意义(P<0.05),但预处理手术组尿素氮低于手术组(P<0.05);预处理手术组和手术组血肌酐水平分别为(246±50)μmol/L、(318±81)μmol/L,显著高于对照组的(43±5)μmol/L,差异有统计学意义(P<0.05),但预处理手术组低于手术组(P<0.05)。预处理手术组和手术组病理损伤评分分别为(2.4±0.7)分、(3.0±0.6)分,其余3组数为0分,但预处理手术组肾脏损伤程度低于手术组(P<0.05)。结论丁苯酞预处理对缺血/再灌注性AKI大鼠模型肾功能具有一定的保护作用。
Objective To study the protective effect of the preventive application of butylphthalide on ischemia reperfusion renal injury in SD rats.Methods A total of 60 SD rats were randomly divided into con-trol group,pretreatment group（simple butylphthalide gavage）,sham operation group,pretreatment operation group（ butylphthalide gavage and double renal artery clipping operation ） , operation group （ single pair of renal artery clipping operation） by random arrangement table,with 12 rats in each group.Rats in pretreat-ment group and pretreatment operation group were given butylphthalide 80 mg/（ kg？ d ） to gavage for 2 weeks before modelling,to prepare SD rat model of renal ischemia reperfusion acute renal injury.Changes of blood creatinine,uremic nitrogen at 24 h of ischemia reperfusion of all the groups were detected,and renal pathological results were observed by hematoxylineosin staining .Results Urea nitrogen levels of the sham operation group,pretreatment operation group and operation group were （8.9 ±1.6） mmol/L,（33.2 ±7.7） mmol/L,（44.9 ±17.3） mmol/L,significantly higher than （6.1 ±1.2） mmol/L of the control group,the differences were statistically significant（P 〈0.01）,but the urea nitrogen level of pretreatment operation group was lower than operation group（P〈0.01）.Blood creatinine level of the pretreatment operation group and operation group were （ 246 ±50 ） μmol/L, （ 318 ±81 ） μmol/L, significantly higher than （ 43 ± 5） μmol/L of the control group, the differences were statistically significant（P〈0.01）,but the blood creat-inine level of pretreatment operation group was lower than operation group（P 〈0.01）.Pathological injury scores of the pretreatment operation group and operation group were （2.4 ±0.7） scores,（3.0 ±0.6） scores,significantly higher than 0 score of the control group,the differences were statistically significant（P〈0.01）,but pathological injury score of pretreatment operation group was lower than operatio
出处
《医学综述》
2015年第10期1852-1854,共3页
Medical Recapitulate
关键词
缺血/再灌注损伤
急性肾损伤
丁苯酞
Ischemia-reperfusion injury
Acute kidney injury
Butylphthalide
