摘要
目的观察血管紧张素Ⅱ(AⅡ)/心房肽(ANP)比值失衡在烧伤后急性肾损伤(acuterenalin-jury,ARI)发病机制中的重要作用,探讨丹参及β-七叶皂甙钠通过调节AⅡ/ANP比值来减轻肾内血管收缩所引起的肾功能损害的治疗作用及其机制,为临床治疗烧伤后ARI提供理论依据。方法制作30%TBSAⅢ度大鼠烫伤模型,并随机分盐水组(M)、丹参组(S)、β-七叶皂甙钠组(A)及联合组(C,丹参+β-七叶皂甙钠)。另设假烫对照组(N),每组8只。烫伤后24h取静脉血测血清BUN、Cr、Na及尿Cr、Na,检测血浆及肾组织血管紧张素Ⅱ(AⅡ)、心房肽(ANP)含量。结果盐水组及治疗各组血浆及肾组织AⅡ、ANP含量均增高,盐水组AⅡ增加幅度明显高于ANP增加幅度,存在明显AⅡ/ANP比值失衡。3个治疗组AⅡ增高幅度明显降低,与盐水组相比AⅡ/ANP比值显著降低,联合组最低。结论AII/ANP失衡引起肾血管持续收缩,导致肾缺血性损伤,可能参与了烧伤后肾小球滤过功能及肾小管回吸收功能的损害。丹参及β-七叶皂甙钠通过降低AⅡ/ANP的比值,减轻肾内血管的收缩,从而提高肾小球滤过率和改善肾小管的回吸收功能,对烧伤后肾损伤有一定防治作用,联合应用效果更佳。
[Objective] To observe the role of A Ⅱ/ANP ratio imbalance in the pathogenesis of acute renal injurY after burn. A theoretical basis for elinical treatment of acute renal injury was provided. [Methods] Forty rats were randomly divided into sham control （N, n =8）, sodium chloride group （M, n =8）, salviae mihrorrhizae group （S, n =8）, β-aescinom natricum group （A, n =8）, and combination group （C, n =8）. The rats in M, S, A and C groups were sub- jected to 30% TBSA Ⅲ degree scald on the back. The blood and kidney samples were harvested from the rats at 24 PBH for the determination of BUN, Cr, Na in blood serum and Cr, Na in urine, angiotensin Ⅱ （AⅡ） and atrial natriuretic peptide （ANP） contents in Plasma and renal tissue. [Results] The contents of A Ⅱ and ANP in plasma and kidney are elevated in M group and each treatment groups. In M group, AⅡ increase significantly higher than the increase of ANP. There is an obvious A Ⅱ/ANP ratio imbalance. Compared with those in M group, the increased range of A Ⅱ and AⅡ/ANP ratio were decreased significantly in three treatment groups. Espeeially in C group. [Conclusion] A Ⅱ/ANP ratio imbalance cause continued contract of the renal vascular rand and lead to renal ischemic injury. It played an important role in the pathogenesis of post-burn renal damage. A Ⅱ/ANP ratio were decreased and the intrarenal vascular contraction were reduced by either Salviae Miltrorrhizae or β-aescinom natricum. Two drugs might improve the glomerular filtration rate and the return absorption of renal tubule. The results indicated that all the agents used might be effective in prevention and treatment of postburn renal injury, especially when they used together.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第9期1290-1293,1297,共5页
China Journal of Modern Medicine
