摘要
[目的]探索人胚胎嗅鞘细胞（hOECs）移植修复大鼠脊髓全横断损伤的可行性。[方法]分离、培养并鉴定人胚嗅鞘细胞，24只Wistar大鼠，随机分为实验组和对照组，均行T10节段脊髓全横断。术后第9～10d，实验组、对照组分别移植Hoechst33342标记的hOECs 5μl（2．5×10^5个细胞）、DMEM—F12培养基5μl。移植术后第1、2、4、6、8、10周进行BBB运动功能评分，取材行荧光化学（Hoechsd3342）和免疫组织化学染色（P75、NF-200、Synaptophysin）。双盲条件下进行数据统计。[结果]移植的hOECs可以在损伤脊髓内存活10周以上，可向损伤脊髓头尾两端迁移；术后4～10周实验组的BBB运动功能评分明显高于对照组（P〈0．01）；P75染色实验组呈阳性反应；NF-200和Synaptophysin免疫组化染色，实验组神经纤维、突触数目和密度都较对照组高。[结论]hOECs移植对脊髓全横断损伤大鼠运动功能恢复具有促进作用。
[ Objective] To investigate the therapy of human enbryonic olfactory ensheathing cells（hOECs） transplantation on adult rats＇ transected spinal cord. [ Method] Separated, cultured hOECS from abortus were obtained and percent age of P75 positive ceils was calculated. Twenty-four Wistar rats were choosen and divided into experimental group and control group randomly. All rats of two goups were transected at the T10 segment of spinal cord. Nine to ten days later 5 ul（2.5 × 10^5） hOECs （labled by Hoechst 33342） were injected in the contusion area of lesion site in transplantation group. In control group same volume of DMEM-FI2 were transplanted. 1,2,4,6,8 and 10 weeks later all rats were estimated by system of the BBB locomotion score respectively. Then part of them were killed. The tissue section of spinal cords were separated and inspected by fluorescence microscope or the methods of immunohistochemical staining. Data were processed with statistical tools in double-blinded condition. [ Result] Transplanted hOECs can survive for at least ten weeks and even migrated from the injuried area in spinal cord. From 4 weeks to 10 weeks the BBB locomotion scores of experimental group were improved significantly contrasting to that of control group （P 〈0.01 ）. P75 immunohistochemical staining showed the experimental group had a positive reaction. The number of nerve fibers and synapse were observed more than that of control group. [ Conclusion]Transplantion of hOECs can enhance recovery of locomotion function for adult rats with spinal cord injury.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第14期1093-1095,i0002,共4页
Orthopedic Journal of China
基金
云南省自然科学基金资助项目(2002C0070M)
关键词
脊髓损伤
人胚胎嗅鞘细胞
细胞移植
BBB评分
Spinal cord injury
Human embryonic olfactory ensheathing cells
Cell transplantation
BBB locomotion score
