摘要
目的 :研究脊髓损伤后脊髓内自由基丙二醛 (MDA)含量及超氧化物岐化酶 (SOD)的活性变化 ,以探讨自由基在脊髓继发性损伤中的作用机制。方法 :4 2只健康家兔采用改良式Allen氏法造成脊髓损伤模型 ,测定其损伤后 12h、4 8h、3d、7d、14d及 2 1d脊髓组织内自由基含量和超氧化物岐化酶的活性。结果 :脊髓损伤后 ,MDA在 2 4h内显著升高 ,7d达最高峰 ,升幅达 90 % ,并随之逐渐下降 ;伤后SOD活性显著下降 ,3d时达最低 ,降幅达 5 4 % ,随后并逐渐回升。结论 :自由基和超氧化物歧化酶参与了脊髓继发性损伤的病理过程。
Objective:To observe the active change of malonyldialdehyde (MDA)and superoxide dissmutas(SOD)contents in a series of intervals after acute spinal cord injury(SCI),and to study the free radical variation in secondary pathological damage of the spinal cord.Methods:The SCI model in rabbits were made with modified Allen’s method.The amount of MDA and SOD of spinal cord tissue were determined in the 12 h , 48 h , 3 d ,7 d,14 d and 21 d after injury.Results:The concentration of MDA significantly increased in the 24 h after SCI,reached the peak at the 7 d and then dropped down gradually.The activity of SOD significantly descended after SCI,was the lowest at 3 d and then return slightly.Conclusion:This study suggested that free radical plays an important role in the secondary pathological damage of SCI.
出处
《中国骨伤》
CAS
2004年第7期391-392,共2页
China Journal of Orthopaedics and Traumatology
关键词
脊髓损伤
自由基
超氧化物岐化酶
病理学
Spinal injuries
Free radical
Superoxide dissmutas
Animals,laboratory
