摘要
目的观察酒精性脂肪肝大鼠肝组织TNF-αmRNA和IL-6mRNA的表达、血清TNF-α和IL-6的含量、脂质过氧化损伤等的变化情况,探讨酒精性脂肪肝的发病机制。方法23只SD大鼠随机分为正常组10只和模型组13只,以50%酒精灌胃和自由饮用10%酒精饮料进行酒精性脂肪肝的造模,连续14周后观察肝组织的病理学改变,血清TG、TC、ALT、AST、FFA、MDA、SOD、GSH-PX、TNF-α、IL-6的含量以及肝组织TNF-α、IL-6mRNA的表达情况。结果模型组大鼠肝组织出现不同程度的脂肪变,血清TG、FFA、MDA、TNF-α和IL-6含量均较正常组显著升高,而SOD、GSH-PX含量则较正常组显著降低;肝组织TNF-αmRNA、IL-6mRNA表达较正常组显著增强。结论长期单纯大量饮酒可造成大鼠脂质代谢系统的紊乱,形成酒精性脂肪肝,同时出现氧化应激/脂质过氧化反应的增强和TNF-α、IL-6等细胞因子分泌的异常。
Objective To measure the the expression of TNF - α,IL - 6mRNA in liver of rats with alcohol fatty liver, and the amounts of TNF - αand IL - 6, to observe the lesion of lipid superoxidation, to explore the pathogenesis of alcohol fatty liver. Methods 23 of SD rats were divided into the normal group（10） and the model group（ 13 ） ,all the rats in the model were given 50% alcohol by daily gavage, and drank 10% alcohol, after 14 weeks, all the rats were killed and the pathological change of liver were observed, the amounts of serum TG,TC, ALT, AST, FFA, MDA, SOD, GSH - PX, TNF - αand IL - 6 were examined, the expression of TNF - α, IL - 6mRNA was measured. Results In the model ,to different degree, there were some fatty steatosis , the amounts of TG,FFA,MDA,TNF - αand IL - 6 were higher than that in the normal group, but the amounts of SOD and GSH - PX lower, in comparison with the normal, the expression of TNF - αmRNAand IL - 6mRNA in liver of model were significantly increased. Conclusions long - term of ingestion of potation maybe cause the disorder of lipid metabolic system and alcohol fatty liver came into being. Simultaneously with the enhancement of oxidation stress/lipid superoxidation and abnormity of secretion of some cytokine such as TNF -αand IL - 6.
出处
《医学研究杂志》
2006年第7期18-20,共3页
Journal of Medical Research
