摘要
为了解决高氧预适应（ＨｙｐｅｒｏｘｉｃｐｒｅｃｏｎｄｉｔｉｏｎｉｎｇＨＯＰ）对大鼠心肌缺血再灌注时自由基的影响，本实验将实验组大鼠放入高压氧舱内，每日吸８０－８５％氧气（１ａｔｍ）６ｈ，连续７ｄ。利用Ｌａｎｇｅｎｄｏｒｆ装置做成心肌缺血再灌注模型，采用电子自旋共振技术测定自由基含量。实验动物随机分为二组，第一组为对照组：缺血１０ｍｉｎ，再灌注６０ｍｉｎ。第二组为ＨＯＰ组：缺血１０ｍｉｎ，再灌注６０ｍｉｎ。实验观察冠脉回流液中自由基ＰＢＮ加合物含量。结果表明：在再灌注过程中，１、５、１０ｍｉｎ３个时间点，ＨＯＰ组ＰＢＮ加合物含量较对照组明显减少。提示：ＨＯＰ能减少缺血再灌注时自由基的产生。
In order to observe the effect of hyeroxic preconditioning(HOP) on free radical(FR) generated by ischemia reperfusion, rats were exposed to 80-85% O 2(1 atm) six hours a day for 7 days. The control rats were exposed to room air. Both groups were killed and their hearts were isolated and under gone 10 min ischemia followed by 60 min reperfusion by Langendorff method. RP PBN adducts were measured by electron spin resonance(ESR) at baseline, 1, 5, and 10 min of reperfusion. The results showed that PBN adducts in HOP group were significantly less than that in control group. It is concluded that oxygen free radicals were reduced by HOP during myocardial ischemia reperfusion in rats.
出处
《中国应用生理学杂志》
CAS
CSCD
1996年第3期227-229,共3页
Chinese Journal of Applied Physiology
关键词
高氧预适应
心肌缺血
再灌注损伤
自由基
hyeroxic preconditioning(HOP)
ischemia reperfusion injury
free radical
ESR
