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高氧预适应增强大鼠心肌Mn-SOD基因表达及缺血梗塞心肌SOD活力 被引量:2

EFFECTS OF HYPEROXIC PRECONDITIONING ON THE EXPRESSION OF MnSOD GENE AND THE SOD ACTIVITYIN ISCHEMIC AND INFARCT MYOCARDIUM OF RATS
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摘要 本文报告了一种新的心脏预适应途径即高氧预适应(hyperoxicpreconditioning,HOP)。为观察高氧预适应对鼠心肌抗氧化酶的影响,我们给大鼠吸入80%~85%氧气,1atm,每天6h,连续7d(HO组),然后进行实验并与室内空气环境下喂饲7d的大鼠(对照组)进行比较。30只Wister大鼠随机分为6组,每组5只:HO-1和对照-1组,HO吸入结束后直接处死,采用Northen杂交法测定心肌Mn-SODmRNA表达;HO-2和对照-2,采用Langendorf法造成60min缺血,60min再灌注,测心肌SOD与冠脉回流液的CPK及SOD活力;HO-3和对照-3,同法造成10min缺血,60min再灌注,测心肌与冠脉回流液的SOD活力。结果显示,HO-1组心肌Mn-SODmRNA表达明显较对照组增强。缺血及梗塞心肌SOD的活力分别为(%/g):HO-283.6±0.5;对照-276.7±1.7(P<0.01),HO-390.1±1.9;对照-385.1±1.4(P<0.05)。HO组冠脉回流液的SOD活力在缺血再灌注期明显高于对照组(P<0.001)。冠脉回流液CPK活力HO-2组再灌注30及? e report a new method of myocardial preconditioning named hyperoxic preconditionning(HOP). In order to study the effects of HOP on myocardial ischemic injury and antioxidant enzymes, the adult rats were preexposed to 80%~85% of oxygen, 1 atm, six hours per day for seven days before the experiments (HO groups). The control groups were in room air for seven days. Thirty Wister rats were devided randomly into six groups (n=5 for each group): the rats were killed afier the HO protocol and the expressions of MnSOD mRNA in myocardium were determined by Nonhern Blot (HO1 and controll); the isolated rat hearts with Langendorff method underwent 60min ischemia followed by 60min reperfusion (HO2 and control2) or 10min ischemia followed by 60min reperfusion(HO3 and control3). The activities of SOD in myocardium and in coronary efluent were measured in the last four groups,the activities of CPK in coronary efluent were measured in HO2 and control2 groups. The results showed that the expressions of MnSOD mRNA in myocardium were stronger in HOl group than that in controll group; the activities of SOD were significantly higher in HO2 and HO3 groups than those in control groups. The activities of CPK in coronary effluents were significantly lower in HO2 than those in control2 group at 30 and 60 min after reperfusion (P<0.05). Conclusion: hyperoxic preconditioning(HOP) can induce the increased expression of MnSOD mRNA and the activities of SOD in myocardium in rat.
出处 《中国应用生理学杂志》 CAS CSCD 1998年第3期245-249,共5页 Chinese Journal of Applied Physiology
关键词 高氧预适应 大鼠 心肌 MN-SOD 心肌病 hyperoxic preconditioning rat myocardium MnSOD
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