摘要
目的建立益肾灵颗粒(无蔗糖)中淫羊藿苷含量的HPLC测定方法。方法采用C18柱;以乙腈-水(30∶70)为流动相;流速为1.0mL/min;柱温为30℃;检测波长为270nm。结果淫羊藿苷在4.1～20.5μg范围内有良好的线性关系,回归方程Y=25265.80X+7891.08,r=1,平均回收率为102.90%,RSD=1.72%。结论本法操作分离效率高、稳定性好、分析速度快,为制剂的质量控制提供了可靠的方法。
Objective To establish a method for determination of Icariin in Yishengling granules （Sucrose-free） by HPLC. Methods Using a column packed with C18 and mixture of acetonitrile-water （30 ＂ 70） as the mobile phase. The flow rate was 1.0 mL/min and the temperature was 30℃. The detection wavelength was at 270 nm. Result The linear range of Icarlin was 4.1-20.5μg （r=1）. The mean recovery was 102.90% and RSD was 1.72%. Conclusion The method is simple, reliable and rapid, and can be applied to the quality control of Yishenling granules （Sucrose-free）.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第7期46-47,共2页
Chinese Journal of Information on Traditional Chinese Medicine
