摘要
目的:建立益母草颗粒(无蔗糖型)中盐酸水苏碱含量的测定方法。方法:采用反相高效液相色谱法,Ultimate Column XB-NH2(250 mm×4.6 mm,5μm)为色谱柱,乙腈-水(90∶10)为流动相,检测波长为192 nm,流速为1.0 ml/min。结果:盐酸水苏碱在0.964～19.280μg范围内与峰面积呈良好线性关系(r=0.999 3),盐酸水苏碱平均加样回收率为97.7%,RSD为1.23%(n=6)。结论:本方法可靠性高,准确性和重复性好,适用于益母草颗粒(无蔗糖型)中盐酸水苏碱的含量测定。
Objective： To develop a method for determination of Stachydrine Hydrochloride in Herba Leonuri Granules （sugar free）. Methods： The analytical column of reversed-phase high performance liquid chromatography （HPLC） method was Ultimate Column XB-NH2 （250 min×4.6 mm, 5 μm）. The mobile phase was a mixture of acetonitrile-water （90：10）, the flow rate was 1 ml/min. The detector wavelength was set at 192 nm. Results： The calibration curve was linear over the range of 0.964-19.280 μg for Staehydrine Hydrochloride （r=0.999 3）. The average recovery of Stachydrine Hydrochloride was 97.7%, RSD=1.23% （n=6）. Conclusion： The method is convenient, economical, it is adapted for use in the quantitative analysis of Stachydrine Hydrochloride.
出处
《中国医药导报》
CAS
2011年第26期70-72,共3页
China Medical Herald
关键词
盐酸水苏碱
反相高效液相色谱法
益母草颗粒(无蔗糖型)
Staehydrlne Hydrochloridein
Reversed-phase high performance liquid chromatography
Herba Leonuri Granules （sugar free）
