摘要
目的 采用高效液相色谱法测定脑灵素片中淫羊藿苷的含量,以控制该制剂的质量。方法 以C18化学键合硅胶柱分离淫羊藿苷,乙腈-0.075 mol·L-1磷酸溶液(用三乙醇胺调节pH 6.4)(27:73)为流动相,UV检测波长270 nm。结果 淫羊藿苷峰与其他组分峰的分离度为5.3,理论塔板数以淫羊藿苷峰计算为6 500;方法的平均加样回收率为98.5％,RSD为0.8％(n=5),淫羊藿苷进样量与吸收面积分值呈良好的线性关系。线性范围0.25-2.5μg。结论 该法测定脑灵素片中淫羊藿苷的含量,结果准确,重复性好。
OBJECTIVE To dominate the quality of Naolingsu tablets, the content of Lcariin was determined byRP-HPLC. METHODS Lcariin was separated on spherisorb C18 column by acetonitrile-0. 075 mol · L -1 phosphoricacetic (adjusted pH=6. 4 by using triethabolamine) (27 : 73) as the mobile phase; the detection wavelength was 270nm. RESULTS The resolution was 5. 3. The number of theoretical plates calculated Lcariin forpeak was 6 500. Theaverage recovery was 98. 3% and RSD was 0. 8% (n = 5). There was a good linear relationshipbetween the concen-tration of Lcariin and absorption area value in the range of 0. 25 - 2. 5 μg. CONCLUSIONS Themethod was highly re-producible and accurate.
出处
《中南药学》
CAS
2004年第6期348-350,共3页
Central South Pharmacy
