摘要
目的建立茄病镰刀菌感染皮肤的ICR小鼠模型。方法将茄病镰刀菌三个不同浓度的菌悬液分别接种于正常和免疫抑制ICR小鼠受损及正常未受损的皮肤,于接种后第7、14、21、28天处死动物,取皮疹和各脏器进行真菌镜检、培养和组织病理学检查。结果正常和免疫抑制组皮肤受损的小鼠均出现皮疹,而免疫抑制组皮疹程度重,持续时间长,且真菌镜检、培养及组织病理学大都可见菌丝。皮肤未受损的小鼠不发生皮疹。实验小鼠均未出现系统播散。结论接种109CFU/mL或1010CFU/mL茄病镰刀菌的孢子悬液于免疫抑制ICR小鼠擦伤的皮肤,可有效造成ICR小鼠的茄病镰刀菌皮肤感染。
Objective To establish a murine model with local skin infection caused by Fusarium solani. Methods Fusarium solani suspension in three different concentrations were inoculated on the intact or abrated skin of normal or immunocompromised ICR mice. Histopathologic examination and fungal culture were performed on 7th d, 14th d, 21st d and 28th d after inoculation, respectively. Results The lesions were developed on abrated skin of all normal and immunocompromised mice, and were more severe and persisted longer in immunocompromised mice than those in normal mice. Moreover, hyphae appeared mostly in histopathologic examination and fungal culture. No lesion was found on undamaged skin. No disseminated infection of Fusarium solani was revealed in all tested mice. Conclusion Inoculation of Fusarium solani cell suspension in concentration of 10^9 or 10^10 CFU/ml on the abrated 0skin of immunocompromised ICR mice can effectively generate a mouse cutaneous Fusarium solani infection model, and to serve experimental research on dermatological infection of this fungus.
出处
《中国实验动物学报》
CAS
CSCD
2006年第2期104-106,I0007,共4页
Acta Laboratorium Animalis Scientia Sinica
关键词
镰刀菌属
皮肤
模型
动物
Fusarium solani
Skin
Model, mouse
