摘要
在新鲜分离的大鼠背根神经节（ＤＲＧ）神经元应用全细胞膜片钳技术记录并证实了膜受体的存在，然后将此同一细胞吸入一较大口径的微吸管，再转移至载玻片上，在倒置显微镜下完成细胞的固定、漂洗及免疚组织化学抗原－抗体反应和显色等步骤。应用这一方法成功地在单个ＤＲＧ神经元膜上确证了Ｎ－甲基－Ｄ－天冬氨酸及Ｐ物质自身受体的存在。
A new method combining immunohistochemical revelation of intracellular neurotransmitterand electrophysiological identification of membrane receptors in the same neuron freshly isolatedfrom rat dorsal root ganglion(DRG)is reported.In brief,after identification of the existence ofreceptor in the membrane of the neuron by whole-cell patch clamp technique,the neuron wassucked into a large micropipette and then transferred onto a slide,on which the fixation,rins-ing,antigen-antibody reaction and staining of the preparation were done by conventionalimmunohistochemical procedures.Recently,both NMDA and SP receptors as autoreceptors inthe membrane of isolated rat DRG neuron were successfully identified by this new method.
出处
《中国应用生理学杂志》
CSCD
1996年第1期77-81,共5页
Chinese Journal of Applied Physiology
关键词
神经电生理
神经递质
背根神经节
神经元
whole-cell patch-clamp technique
immunohistochemistry
DRG neuron
NMDAreceptor
SP receptor
autoreceptor
