摘要
目的:研究香叶天竺葵油(PLO)的化学表征,寻找抗氧化作用的物质基础。方法:测定PLO体外抑制豚鼠心、肝、肾脂质过氧化、清除O.2、.OH、二苯代苦味基自由基(DPPH.)和抑制H2O2诱导的红细胞氧化溶血的作用,用气相色谱法对PLO进行化学表征,测定其单体对DPPH.的清除作用。结果:经方差分析表明,PLO对豚鼠肝、肾、心自发脂质过氧化、CC l4诱导的肝脂质过氧化及中高剂量抑制H2O2诱导的红细胞溶血作用有极显著的(P<0.01)抑制作用。对O.2和DPPH.的清除作用与阴性对照相比有极显著的差异(P<0.01)。气相色谱和单体抗氧化实验证实占PLO组分30.06%的香茅醇抗氧化能力与PLO接近。结论:PLO有抗氧化能力,香茅醇是主要抗氧化成分。
AIM： To study medical effect of the antioxidation activity of essential oil from smell storusbill （Pelargonium graveolens, L＇ Her）（PLO） METHODS： Free radicals（O ·2、· OH and DPPH· ）scavenging activities were investigated in different special chemical systems. Its anti-lipoperoxidantion activity was studied with MDA generation model in vitro. The effect of the hemolysis of cavy RBC induced by H2O2 was also studied. The components of PLO was identified by GC-FID. RESULTS： It was found that PLO showed higher anti-lipoperoxidantion on MDA generation spontaneously in homogenates of cavy heart,liver and kidney. There were significant differences on lipid peroxidant of the cavy liver homogenate induced by CC14 between control group and PLO. There were higher scavenging effect of the extracts of foliage on O · 2 and DPPH · PLO could strongly inhibit the hemolysis of cavy RBC induced by H2O2. The result of GC-FID and DPPH · scavenging activities indicated that the antioxidantion of citronellol was similar with PLO. CONCLUSION： PLO is a good antioxidation and citronellol is the main antioxidated substance in PLO.
出处
《中成药》
CAS
CSCD
北大核心
2006年第6期855-860,共6页
Chinese Traditional Patent Medicine
关键词
香叶天竺葵油
抗氧化
化学表征
essential oil from Pelargonium graveolens. L＇ Her
antioxidation
chemical characterization
