摘要
目的:探讨氯化甲基汞(mthylmercury chloride,MMC)对发育大鼠小脑组织蛋白激酶C(PKC)活性的影响。方法:实验组母鼠从妊娠前90 d至仔鼠出生后30 d连续喂饲含有不同剂量MMC(0.75、1.50和3.00 mg.kg-1)的普通饲料,取其生后(Postnatal day,PND)3、7、14、21和30 d仔鼠小脑组织提取胞浆和胞膜PKC。采用改良Takai法观察MMC对发育大鼠小脑PKC活性的影响。结果:实验组生后不同时间仔鼠小脑组织胞浆和胞膜PKC活性均高于相应对照组,其中实验组Ⅰ(0.75 mg.kg-1MMC)PND 3、7和14 d,实验组Ⅱ(1.5 mg.kg-1MMC)和实验组Ⅲ(3.0 mg.kg-1MMC)仔鼠小脑组织胞浆和胞膜PKC活性明显高于对照组(P<0.05)。结论:MMC可能通过影响大鼠脑发育过程中PKC活性介导其神经毒性作用。
Objective To study the effects in rat developing cerebellum. Methods of methylmercury chloride （MMC） on protein kinase C （PKC） activities The animals in experimental groups were fed standard rat chow with 0. 75 mg MMC （Exp Ⅰ）, 1.5 mg MMC （ExpⅡ） and 3.0 mg MMC （Expm） respectively for 90 d before gestation to 30 d post parturition. Cerebella of pups from each group on postnatal days （PND） 3, 7, 17, 21 and 30 were dissected. All the samples were separated into cytosol and membrane subeellular fractions and assayed for PKC activity by the improved method from Takai＇s. Results Membrane and cytosolic PKC activities of pups＇ cerebella from certain experimental groups were significantly higher than those of corresponding control group, PKC activities of rats from Exp Ⅱ , Exp Ⅲ and PND 3, 7, 14 in Exp Ⅰwere significantly higher than those in control group （P〈0.05）. Conclusion The damage of the rat developing cerebellum by chronic exposure to MMC is possibly correlative with the enhanced effect of MMC on PKC activity.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2006年第3期440-442,共3页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(300706465)
吉林大学青年教师基金资助课题(419070210048)
关键词
甲基汞化合物/毒性
蛋白激酶C
汞中毒
神经系统
动物
实验
methylmercury compounds/toxicity
protein kinase C
mercury poisoning, nervous system
animal,laboratory
